Construction of TUATinsecta database that integrated plant and insect database for screening phytophagous insect metabolic products with medicinal potential
| Autor: | Wakana Nakane, Natsuki Kaminaga, Yoshikazu Kitano, Maaya Nishiko, Takeru Nakazato, Hidemasa Bono, Richard J. Simpson, Miho Nakano, Takuma Sakamoto, Hiroko Tabunoki, Kaoru Kinoshita, Isao Ogiwara, Hisashi Nakamura, Kikuo Iwabuchi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Citrus Insecta Papilio xuthus Databases Factual Cell Survival media_common.quotation_subject lcsh:Medicine Insect computer.software_genre 010603 evolutionary biology 01 natural sciences Article 03 medical and health sciences Feces Inhibitory Concentration 50 Drug Discovery Inhibitory concentration 50 Animals Humans Poncirus Author Correction lcsh:Science Swallowtail butterfly media_common Cell Proliferation Inflammation Larva Biological Products Multidisciplinary biology Database Drug discovery Frass fungi lcsh:R Hep G2 Cells Human cell biology.organism_classification Plant Leaves 030104 developmental biology lcsh:Q computer Butterflies Entomology HeLa Cells |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Phytophagous insect larvae feed on plants containing secondary metabolic products with biological activity against other predatory organisms. Phytophagous insects can use their specialised metabolic systems to covert these secondary metabolic products into compounds with therapeutic properties useful to mankind. Some Asians drink tea decoctions made from phytophagous insect frass which is believed to be effective against inflammatory diseases. However, insects that can convert plant-derived secondary metabolic products into useful human therapeutic agents remain poorly studied. Here, we constructed the TUATinsecta database by integrating publicly plant/insect datasets for the purpose of selecting insect species. Using TUAT-insecta we selected the Asian swallowtail butterfly, Papilio xuthus larvae fed on several species of Rutaceous plants and examined whether the plant-derived secondary metabolites, especially those present in frass, were chemically altered or not. We extracted metabolic products from frass using three organic solvents with different polarities, and evaluated solvent fractions for their cytotoxic effects against several human cell lines. We found that chloroform frass extracts from P. xuthus larvae fed on Poncirus trifoliata leaves contained significant cytotoxic activity. Our findings demonstrate that screening of insect species using the ‘TUATinsecta’ database provides an important pipeline for discovering novel therapeutic agents that might be useful for mankind. |
| Databáze: | OpenAIRE |
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