Increased expression of protein kinase CK2α correlates with poor patient prognosis in epithelial ovarian cancer

Autor: Xiaojing Wang, Yanfang Li, Ze-Biao Ma, Jianchuan Xia, Jiehua He
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Oncology
endocrine system diseases
Protein Expression
Apoptosis
Carcinoma
Ovarian Epithelial
Biochemistry
0302 clinical medicine
Cell Movement
Medicine and Health Sciences
Small interfering RNAs
Cell Cycle and Cell Division
Neoplasms
Glandular and Epithelial
Casein Kinase II
Ovarian Neoplasms
Regulation of gene expression
Gene knockdown
Multidisciplinary
Cell Death
Cell Cycle
Middle Aged
Cell cycle
Prognosis
female genital diseases and pregnancy complications
Ovarian Cancer
Nucleic acids
Gene Expression Regulation
Neoplastic
Cell Processes
030220 oncology & carcinogenesis
Immunohistochemistry
Medicine
Female
Anatomy
Research Article
medicine.medical_specialty
Science
Nose
Research and Analysis Methods
Cell Line
03 medical and health sciences
Cell Line
Tumor
Internal medicine
Genetics
Gene Expression and Vector Techniques
Biomarkers
Tumor
medicine
Carcinoma
Humans
Non-coding RNA
Molecular Biology Techniques
Molecular Biology
Cell Proliferation
Aged
Neoplasm Staging
Proportional Hazards Models
Molecular Biology Assays and Analysis Techniques
Biology and life sciences
business.industry
Cell growth
Cancers and Neoplasms
Cell Biology
medicine.disease
Gene regulation
030104 developmental biology
Tumor progression
Face
RNA
Gene expression
Cytology
Ovarian cancer
business
Gynecological Tumors
Head
Zdroj: PLoS ONE, Vol 12, Iss 3, p e0174037 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: Epithelial ovarian cancer (EOC) is one of the deadly gynecological malignancies. The function of protein kinase CK2α (CK2α) in EOC is still unknown. Our study aimed to investigate the relationship between the protein expression of CK2α and the tumor progression, the prognosis of human EOC. In this study, we analyzed the expression levels of CK2α through Western blot, using EOC cell lines like A2780, HO8910, COV644, OVCAR3, SKOV3, and the primary normal ovarian surface epithelial (NOSE) cells. Furthermore, OVCAR3 and SKOV3 EOC cells were employed as a cellular model to study the role of CK2α on cell growth, migration, invasion, apoptosis, and cell cycle distribution. In addition, we investigated CK2α protein expression in tumor tissues from patients with EOC by immunohistochemistry and analyzed the association between CK2α expression and clinicopathologic parameters and prognosis of EOC patients. And we found that compared with NOSE cells, CK2α protein expression was increased in A2780, HO8910, OVCAR3, and SKOV3 ovarian cancer cell lines. Decreased CK2α expression suppressed OVCAR3 and SKOV3 cell growth and induced more apoptosis. CK2α knockdown using specific siRNAs inhibited migration and invasion ability of OVCAR3 and SKOV3 cells. In addition, high CK2α protein expression was found in 68.4% (80/117) of EOC patients. Increased CK2α expression of was significantly correlated with FIGO staging and peritoneal cytology. Patients with higher CK2α expression had a significantly poorer overall survival compared with those with lower CK2α expression. Multi-variate Cox regression analysis proved that increased CK2α expression was an independent prognostic marker for EOC. Taken together, our data displayed that CK2α may play a role in tumor aggressive behavior of EOC and could be used as a marker for predicting prognosis of EOC patient. High CK2α expression might predict poor patient survival.
Databáze: OpenAIRE
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