The influence of sweet pepper pectin structural characteristics on cytokine secretion by THP-1 macrophages
| Autor: | Georgia Erdmann do Nascimento, Marcel Ivan Ramirez, Sheila M.B. Winnischofer, Marcello Iacomini, Lucimara M.C. Cordeiro |
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| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine food.ingredient Lipopolysaccharide Pectin THP-1 Cells medicine.medical_treatment Interleukin-1beta Biology Polysaccharide Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound Immune system food medicine Humans Secretion THP1 cell line chemistry.chemical_classification Tumor Necrosis Factor-alpha Hydrolysis Macrophages food and beverages Interleukin-10 030104 developmental biology Cytokine chemistry Biochemistry Cytokines Pectins Cytokine secretion Capsicum Food Science |
| Zdroj: | Food Research International. 102:588-594 |
| ISSN: | 0963-9969 |
| Popis: | Pectins can modulate the biological responses interacting directly with immune cells. The observed responses can strongly be affected by polysaccharide structural features. We analyzed the intrinsic activation capacity of native and modified sweet pepper pectin on cytokine secretion by THP-1 macrophages as well as compare their effects in the presence of lipopolysaccharide. Modified pectin was obtained by partial acid hydrolysis which promoted the removal of side chains as well as the reduction of molecular weight and the degree of methyl esterification of native pectin. The results showed that both fractions had no effect on THP-1 viability. Native pectin at 300μg/mL increased TNF-α, IL-1β and IL-10 cytokine secretion by THP-1 macrophages. However, in the presence of lipopolysaccharide, it can attenuate the inflammatory response by reducing the production of the pro-inflammatory cytokines TNF-α and IL-1β and increasing the anti-inflammatory cytokine IL-10, as well as decreasing the TNF-α/IL-10 and IL-1β/IL-10 ratios. The structural modifications caused by acid hydrolysis affected the intrinsic activation capacity of native pectin to modulate the cytokines secretion. These results indicate that degree of methyl esterification, molecular weight and presence of side chains are important structural features of pectins involved in the modulation of cytokine secretion by THP-1 macrophages. |
| Databáze: | OpenAIRE |
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