Feasibility study of the optical imaging of a breast cancer lesion labeled with upconversion nanoparticle biocomplexes
| Autor: | V. A. Semchishen, Andrei V. Zvyagin, Ekaterina A. Grebenik, Alexey Popov, Evgeny V. Khaydukov, Alla N. Generalova, Andrei V. Nechaev, Varun K. A. Sreenivasan, Dmitry V. Klinov, V. I. Sokolov, Annemarie Nadort, Vladislav Ya. Panchenko, Vitaly P. Zubov, A. S. Akhmanov, Sergey M. Deyev |
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| Přispěvatelé: | Biomedical Engineering and Physics |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Materials science
Receptor ErbB-2 Biomedical Engineering Immunoglobulins Nanoparticle Breast Neoplasms Nanotechnology CHO Cells Adenocarcinoma biomedical optical imaging Models Biological Imaging phantom Biomaterials tissue phantom Cricetulus Breast cancer breast cancer SDG 3 - Good Health and Well-being mini-antibody (wild type) Cell Line Tumor Cricetinae upconversion nanoparticle medicine Animals Humans Luminescent Agents biology Phantoms Imaging Optical Imaging human epidermal growth factor receptor 2 Antibodies Monoclonal Cancer medicine.disease Atomic and Molecular Physics and Optics Photon upconversion Electronic Optical and Magnetic Materials Molecular Probes biology.protein Feasibility Studies Nanoparticles Female Barstar Carrier Proteins Preclinical imaging Biomedical engineering |
| Zdroj: | Journal of biomedical optics, 18(7). SPIE Grebenik, E A, Nadort, A, Generalova, A N, Nechaev, A V, Sreenivasan, V K A, Khaydukov, E V, Semchishen, V A, Popov, A P, Sokolov, V I, Akhmanov, A S, Zubov, V P, Klinov, D V, Panchenko, V Y, Deyev, S M & Zvyagin, A V 2013, ' Feasibility study of the optical imaging of a breast cancer lesion labeled with upconversion nanoparticle biocomplexes ', Journal of Biomedical Optics, vol. 18, no. 7, 076004 . https://doi.org/10.1117/1.JBO.18.7.076004 |
| ISSN: | 1083-3668 |
| DOI: | 10.1117/1.JBO.18.7.076004 |
| Popis: | Innovative luminescent nanomaterials, termed upconversion nanoparticles (UCNPs), have demonstrated considerable promise as molecular probes for high-contrast optical imaging in cells and small animals. The fea- sibility study of optical diagnostics in humans is reported here based on experimental and theoretical modeling of optical imaging of an UCNP-labeled breast cancer lesion. UCNPs synthesized in-house were surface-capped with an amphiphilic polymer to achieve good colloidal stability in aqueous buffer solutions. The scFv4D5 mini-anti- bodies were grafted onto the UCNPs via a high-affinity molecular linker barstar:barnase (Bs:Bn) to allow their spe- cific binding to the human epidermal growth factor receptor HER2/neu, which is overexpressed in human breast adenocarcinoma cells SK-BR-3. UCNP-Bs:Bn-scFv4D5 biocomplexes exhibited high-specific immobilization on the SK-BR-3 cells with the optical contrast as high as 10∶1 benchmarked against a negative control cell line. Breast cancer optical diagnostics was experimentally modeled by means of epi-luminescence imaging of a mono- layer of the UCNP-labeled SK-BR-3 cells buried under a breast tissue mimicking optical phantom. The experimental results were analyzed theoretically and projected to in vivo detection of early-stage breast cancer. The model pre- dicts that the UCNP-assisted cancer detection is feasible up to 4 mm in tissue depth, showing considerable potential for diagnostic and image-guided surgery applications. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. (DOI: 10 .1117/1.JBO.18.7.076004) |
| Databáze: | OpenAIRE |
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