Transmission of Sheep Scrapie to Elk (Cervus Elaphus Nelsoni) by Intracerebral Inoculation: Final Outcome of the Experiment
| Autor: | Juergen A. Richt, Amir N. Hamir, Janice M. Miller, Robert A. Kunkle, Melanie J. Chaplin, Randall C. Cutlip, Allen L. Jenny, M.J. Stack |
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| Rok vydání: | 2004 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Prions 040301 veterinary sciences animal diseases Central nervous system Scrapie Diagnosis Differential 0403 veterinary science 03 medical and health sciences Western blot medicine Animals Rocky Mountain elk Sheep General Veterinary biology medicine.diagnostic_test Wasting Syndrome Inoculation Deer Brain 04 agricultural and veterinary sciences Chronic wasting disease biology.organism_classification medicine.disease Spinal cord Immunohistochemistry Virology 030104 developmental biology medicine.anatomical_structure Case-Control Studies Autopsy |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 16:316-321 |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | This is a final report of an experimental transmission of sheep scrapie agent by intracerebral inoculation to Rocky Mountain elk ( Cervus elaphus nelsoni). It documents results obtained in experimental ( n = 6) and control ( n = 2) elk. During the first 2 years postinoculation (PI), 3 animals died or were euthanized because of infection or injuries other than spongiform encephalopathy (SE). In years 3 and 4 PI, 3 other inoculated elk died after brief terminal neurological episodes. Necropsy of these animals revealed moderate weight loss but no other gross lesions. Microscopically, characteristic lesions of SE were seen throughout the brain and spinal cord, and the tissue was positive for proteinase K-resistant prion protein (PrPres) by immunohistochemistry (IHC) and by Western blot. Scrapie-associated fibrils (SAF) were observed by negative-stain electron microscopy in the brain of elk with neurologic signs. PrPres and SAF were not detected in the 3 inoculated elk necropsied during the first 2 years or in the 2 control animals. Retrospective analysis of the gene-encoding cervid PrP revealed a polymorphism at codon 132. The elk with SE were either homozygous (MM) or heterozygous (LM). These findings confirm that intracerebral inoculation of sheep scrapie agent results in SE with accumulations of PrPres in the central nervous system of elk. Based on morphologic and IHC findings, the experimentally induced SE cannot be distinguished from chronic wasting disease of elk with currently available diagnostic techniques. |
| Databáze: | OpenAIRE |
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