Impaired activation-induced cell death promotes spontaneous arthritis in antigen (cartilage proteoglycan)-specific T cell receptor-transgenic mice
| Autor: | Katalin Olasz, Akos Hegyi, Katalin Mikecz, Oktavia Tarjanyi, Katalin Kis-Toth, Tibor T. Glant, Ferenc Boldizsár |
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| Rok vydání: | 2010 |
| Předmět: |
CD4-Positive T-Lymphocytes
medicine.medical_specialty T cell Immunology Receptors Antigen T-Cell Arthritis Epitopes T-Lymphocyte Mice Transgenic Epitope Article Arthritis Rheumatoid Mice Rheumatology Antigen Internal medicine medicine Immunology and Allergy Cytotoxic T cell Animals Pharmacology (medical) Longitudinal Studies Mice Inbred BALB C Cell Death business.industry Cartilage T-cell receptor Autoantibody medicine.disease Arthritis Experimental medicine.anatomical_structure Endocrinology Proteoglycans Interleukin-4 business Peptides |
| Zdroj: | Arthritis and rheumatism. 62(10) |
| ISSN: | 1529-0131 |
| Popis: | Objective To investigate whether genetic preponderance of a T cell receptor (TCR) recognizing an arthritogenic peptide of human cartilage proteoglycan (PG) is sufficient for development of arthritis. Methods We performed a longitudinal study using BALB/c mice expressing a TCR that recognizes the arthritogenic ATEGRVRVNSAYQDK peptide of human cartilage PG. PG-specific TCR–transgenic (PG-TCR–Tg) mice were inspected weekly for peripheral arthritis until 12 months of age. Peripheral joints were examined histologically, and T cell responses, T cell activation markers, serum cytokines, and autoantibodies were measured. Apoptosis and signaling studies were performed in vitro on T cells from aged PG-TCR–Tg mice. Results Spontaneous arthritis developed as early as 5–6 months of age, and the incidence increased to 40–50% by 12 months of age. Progressive inflammation began with cartilage and bone erosions in the interphalangeal joints, and later expanded to the proximal joints of the front and hind paws. Spontaneous arthritis was associated with a high proportion of activated CD4+ T cells, enhanced interferon-γ and interleukin-17 (IL-17) production, and elevated levels of serum autoantibodies. PG-TCR–Tg mice lacking IL-4 developed arthritis earlier and at a higher incidence than IL-4–sufficient mice. Antigen-specific activation–induced cell death was diminished in vitro in CD4+ T cells of PG-TCR–Tg mice with spontaneous arthritis, especially in those lacking IL-4. Conclusion The presence of CD4+ T cells expressing a TCR specific for an arthritogenic PG epitope is sufficient to trigger spontaneous autoimmune inflammation in the joints of BALB/c mice. IL-4 appears to be a negative regulator of this disease, through attenuation of activation-induced cell death. |
| Databáze: | OpenAIRE |
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