Transcript levels of the eukaryotic translation initiation factor 5A gene peak at early G(1) phase of the cell cycle in the dinoflagellate Crypthecodinium cohnii
| Autor: | D. New, C. M. C. Lam, Joseph T.Y. Wong, K. L. Chan, S. Ghandhi, Francis T. W. Wong |
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| Rok vydání: | 2002 |
| Předmět: |
Eflornithine
Guanine Saccharomyces cerevisiae Molecular Sequence Data Applied Microbiology and Biotechnology chemistry.chemical_compound Eukaryotic translation Peptide Initiation Factors Complementary DNA Polyamines Putrescine Initiation factor Animals RNA Messenger Enzyme Inhibitors Eukaryotic Initiation Factor-5 Phylogeny Toxins Biological chemistry.chemical_classification Hypusine Ecology biology cDNA library G1 Phase Crypthecodinium cohnii biology.organism_classification Blotting Northern Physiology and Biotechnology Trypanocidal Agents Amino acid Blotting Southern Eukaryotic Cells chemistry Biochemistry Dinoflagellida Cell Division Food Science Biotechnology |
| Zdroj: | Applied and environmental microbiology. 68(5) |
| ISSN: | 0099-2240 |
| Popis: | A cDNA encoding a eukaryotic translation initiation factor 5A (eIF-5A) homolog in heterotrophic dinoflagellate Crypthecodinium cohnii (CceIF-5A) was isolated through random sequencing of a cDNA library. The predicted amino acid sequence possesses the 12 strictly conserved amino acids around lysine 52 (equivalent to lysine 50 or 51 in other eukaryotes). A single 1.2-kb band was detected in Northern blot analysis. In synchronized C. cohnii cells, the transcript level peaked at early G 1 and decreased dramatically on the entry to S phase. Although this has not been previously reported, studies of budding yeast ( Saccharomyces cerevisiae ) and certain mammalian cell types suggest a role for eIF-5A in the G 1 /S transition of the eukaryotic cell cycle. Phylogenetic trees constructed with 26 other published eIF-5A sequences suggest that CceIF-5A, while falling within the eukaryotic branches, forms a lineage separate from those of the plants, animals, and archaebacteria. The posttranslational modification of eIF-5A by a transfer of a 4-aminobutyl moiety from spermidine to conserved lysine 50 or 51, forming amino acid hypusine, is the only demonstrated specific function of polyamines in cell proliferation. It has been suggested that polyamines stimulate population growth of bloom-forming dinoflagellates in the sea. We demonstrate here putrescine-stimulated cell proliferation. Furthermore, ornithine decarboxylase inhibitor d -difluoromethylornithine and the specific hypusination inhibitor N -guanyl-1,7-diaminoheptane exhibited inhibitory effects in two species of dinoflagellates. The possible links of polyamines and saxitoxin synthesis to the arginine cycle are also discussed. |
| Databáze: | OpenAIRE |
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