Photoacoustic microscopy of tyrosinase reporter gene in vivo
| Autor: | Arie Krumholz, Sarah J. VanVickle-Chavez, Junjie Yao, William E. Gillanders, Lihong V. Wang, Timothy P. Fleming |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Tyrosinase Biomedical Engineering Biomaterials Melanin Hemoglobins Genes Reporter In vivo medicine Humans Tomography Ultrasonography Melanins Photoacoustic effect Microscopy Reporter gene integumentary system Monophenol Monooxygenase Phantoms Imaging Chemistry Signal Processing Computer-Assisted Equipment Design Transfection JBO Letters Atomic and Molecular Physics and Optics Molecular Imaging Electronic Optical and Magnetic Materials HEK293 Cells Oxyhemoglobins Biophysics sense organs Preclinical imaging Ex vivo |
| Popis: | Photoacoustic tomography is a hybrid modality based on optical absorption excitation and ultrasonic detection. It is sensitive to melanin, one of the primary absorbers in skin. For cells that do not naturally contain melanin, melanin production can be induced by introducing the gene for tyrosinase, the primary enzyme responsible for expression of melanin in melanogenic cells. Optical resolution photoacoustic microscopy was used in the ex vivo study reported here, where the signal from transfected cells increased by more than 10 times over wild-type cells. A subsequent in vivo experiment was conducted to demonstrate the capability of photoacoustic microscopy to spectrally differentiate between tyrosinase-catalyzed melanin and various other absorbers in tissue. |
| Databáze: | OpenAIRE |
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