Biofilm formation in Candida glabrata: What have we learnt from functional genomics approaches?
| Autor: | Guilhem Janbon, Christophe d'Enfert |
|---|---|
| Přispěvatelé: | Biologie et Pathogénicité fongiques, Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Our work on C. glabrata biofilms was funded by grants of Institut Pasteur (PTR50 and PTR173 to CdE and GJ), from the Agence Nationale de la Recherche (ERA-Net Pathogenomics, FUNPATH, ANR-06-PATHO_005-01 to CdE) and the French Government's Investissement d'Avenir program (Laboratoire d'Excellence Integrative Biology of Emerging Infectious Diseases, ANR-10-LABX-62-IBEID to CdE)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-06-PATH-0005,FUNPATH,Genomic approaches to unravel the molecular mechanisms of pathogenicity in the human fungal pathogen candida glabrata(2006), Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris], ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
subtelomeric silencing 030106 microbiology Saccharomyces cerevisiae Mediator Genomics Candida glabrata MESH: Biofilms Epa adhesins PKA pathway Applied Microbiology and Biotechnology Microbiology Chromatin remodeling biofilm MESH: Cell Adhesion 03 medical and health sciences Swi/Snf chromatin remodeling complex MESH: Cell Wall Cell Wall Gene Expression Regulation Fungal Candida albicans Cell Adhesion Animals MESH: Animals MESH: Candida glabrata [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology biology MESH: Genomics Biofilm Candidiasis Computational Biology General Medicine antifungal tolerance biochemical phenomena metabolism and nutrition biology.organism_classification MESH: Candidiasis Cell biology Disease Models Animal Biofilms MESH: Disease Models Animal glucose repression Functional genomics Function (biology) MESH: Gene Expression Regulation Fungal MESH: Computational Biology |
| Zdroj: | FEMS Yeast Research FEMS Yeast Research, Oxford University Press (OUP), 2015, 16 (1), pp.fov111. ⟨10.1093/femsyr/fov111⟩ FEMS Yeast Research, 2015, 16 (1), pp.fov111. ⟨10.1093/femsyr/fov111⟩ |
| ISSN: | 1567-1356 1567-1364 |
| DOI: | 10.1093/femsyr/fov111⟩ |
| Popis: | International audience; Biofilms are a source of therapeutic failures because of their intrinsic tolerance to antimicrobials. Candida glabrata is one of the pathogenic yeasts that is responsible for life-threatening disseminated infections and able to form biofilms on medical devices such as vascular and urinary catheters. Recent progresses in the functional genomics of C. glabrata have been applied to the study of biofilm formation, revealing the contribution of an array of genes to this process. In particular, the Yak1 kinase and the Swi/Snf chromatin remodeling complex have been shown to relieve the repression exerted by subtelomeric silencing on the expression of the EPA6 and EPA7 genes, thus allowing the encoded adhesins to exert their key roles in biofilm formation. This provides a framework to evaluate the contribution of other genes that have been genetically linked to biofilm development and, based on the function of their orthologs in Saccharomyces cerevisiae, appear to have roles in adaptation to nutrient deprivation, calcium signaling, cell wall remodeling and adherence. Future studies combining the use of in vitro and animal models of biofilm formation, omics approaches and forward or reverse genetics are needed to expand the current knowledge of C. glabrata biofilm formation and reveal the mechanisms underlying their antifungal tolerance. |
| Databáze: | OpenAIRE |
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