Targeting Brain Tumors with Mesenchymal Stem Cells in the Experimental Model of the Orthotopic Glioblastoma in Rats
| Autor: | V. A. Ryzhov, Nikol Agadzhanian, Gabriele Multhoff, Grigoriy Timin, Vladimir Deriglazov, Maxim Shevtsov, E. N. Tolkunova, Natalia M. Yudintceva, Anton S. Mazur, Konstantin A Samochernych, Ekaterina Lomert, Natalia Mikhailova |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biodistribution
nonlinear magnetic response QH301-705.5 Cell Medicine (miscellaneous) General Biochemistry Genetics and Molecular Biology Article targeted drug delivery law.invention In vivo Confocal microscopy law multiforme glioblastoma Medicine magnetic resonance imaging Biology (General) biodistribution mesenchymal stem cells business.industry Mesenchymal stem cell Cancer superparamagnetic iron oxide nanoparticles C6 glioma medicine.disease In vitro medicine.anatomical_structure Targeted drug delivery Cancer research business |
| Zdroj: | Biomedicines Volume 9 Issue 11 Biomedicines, Vol 9, Iss 1592, p 1592 (2021) |
| ISSN: | 2227-9059 |
| Popis: | Despite multimodal approaches for the treatment of multiforme glioblastoma (GBM) advances in outcome have been very modest indicating the necessity of novel diagnostic and therapeutic strategies. Currently, mesenchymal stem cells (MSCs) represent a promising platform for cell-based cancer therapies because of their tumor-tropism, low immunogenicity, easy accessibility, isolation procedure, and culturing. In the present study, we assessed the tumor-tropism and biodistribution of the superparamagnetic iron oxide nanoparticle (SPION)-labeled MSCs in the orthotopic model of C6 glioblastoma in Wistar rats. As shown in in vitro studies employing confocal microscopy, high-content quantitative image cytometer, and xCelligence system MSCs exhibit a high migratory capacity towards C6 glioblastoma cells. Intravenous administration of SPION-labeled MSCs in vivo resulted in intratumoral accumulation of the tagged cells in the tumor tissues that in turn significantly enhanced the contrast of the tumor when high-field magnetic resonance imaging was performed. Subsequent biodistribution studies employing highly sensitive nonlinear magnetic response measurements (NLR-M2) supported by histological analysis confirm the retention of MSCs in the glioblastoma. In conclusion, MSCs due to their tumor-tropism could be employed as a drug-delivery platform for future theranostic approaches. |
| Databáze: | OpenAIRE |
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