Annexin expressions are temporally and spatially regulated during rat hepatocyte differentiation
| Autor: | Jacqueline Font, Françoise Chatelet, Jacqueline Weinman, F. Braut-Boucher, Morgane Bomsel, Christiane Guguen-Guillouzo, Bruno Della Gaspera, Serge Weinman |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Annexins Ontogeny Biology Dexamethasone Rats Sprague-Dawley Annexin Pregnancy Internal medicine medicine Animals Annexin A6 RNA Messenger Annexin A5 Glucocorticoids Annexin A2 Cells Cultured Annexin A1 Hepatocyte differentiation Messenger RNA Epidermal Growth Factor Hepatocyte Growth Factor Gene Expression Regulation Developmental Cell Differentiation Cell biology Rats Endocrinology medicine.anatomical_structure Liver Cytoplasm Hepatocytes Immunohistochemistry Female Nucleus Developmental Biology medicine.drug |
| Zdroj: | Developmental dynamics : an official publication of the American Association of Anatomists. 222(2) |
| ISSN: | 1058-8388 |
| Popis: | Annexin (Anx) 1, 2, 5, and 6 expressions were determined at the transcriptional and translational levels in the rat hepatocytes from gestational day 15 to postnatal day 17. Dramatic shifts were observed in Anx 1 and 2 levels, which peaked at day 1 and gestational day 20, respectively, and reached low levels thereafter. However, Anx 5 and 6 rates were more constant. Prenatal administration of dexamethasone (dex) resulted in a decrease of Anx 1 mRNA levels, and a strong increase in Anx 2 mRNA contents. In adult hepatocytes cultured in the presence of EGF or HGF, Anx 1 and 2 expressions resumed. By immunohistochemistry, Anx 1 was detected only in the cytoplasm of hepatocytes of 1- to 3-day-old rats, Anx 2 and 6 both exhibited a redistribution from the cytoplasm toward the plasma membrane, and Anx 5 was present in the nucleus, cytoplasm, and plasma membrane. Thus, Anx 1, 2, 5, and 6 have individual modes of expression and localization in the differentiating hepatocytes, where they might play unique roles at well defined phases of liver ontogeny. © 2001 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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