Heparin and Low Molecular Weight Heparin Decrease Nitric Oxide Production by Human Polymorphonuclear Cells
| Autor: | L. Glaría, Francisco Concepción, Armando Rojas, Gabino Garrido, Dahis Manzanares, Amada Beltrán |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Heparin
Neutrophils medicine.drug_class Low molecular weight heparin General Medicine Adhesion Heparin Low-Molecular-Weight Pharmacology Nitric Oxide In vitro Nitric oxide N-Formylmethionine Leucyl-Phenylalanine chemistry.chemical_compound Polymorphonuclear cells chemistry Biochemistry No synthase medicine Humans No production Methemoglobin medicine.drug |
| Zdroj: | Archives of Medical Research. 30:116-119 |
| ISSN: | 0188-4409 |
| DOI: | 10.1016/s0188-0128(99)00004-4 |
| Popis: | Background Heparin and heparin derivatives with low anticoagulant activity exhibit a wide spectrum of biological functions affecting adhesion, activation and trafficking of leukocytes. Methods We investigated the in vitro effect of heparin and a low molecular weight heparin derivative (LMWH) on nitric oxide (NO) production by human polymorphonuclear leukocytes (PMN). Results N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated NO production was significantly decreased by heparin at doses of 0.5 and 5 μg/mL, while LMWH was only effective at doses of 50 and 200 μg/mL by means of a mechanism not related to NO synthase (NOS) activity. Conclusions These results support the hypothesis that heparin and LMWH derivatives may offer therapeutic benefit for inflammatory diseases where NO plays a protagonic role. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect