In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients
| Autor: | Carl Wood, Alan O Trounson, Annette Kausche |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Infertility
medicine.medical_specialty medicine.drug_class Cleavage Stage Ovum Biology Specimen Handling Human fertilization Internal medicine medicine Humans Cellular Senescence Insemination Artificial Homologous Fetus Embryogenesis Obstetrics and Gynecology medicine.disease Oocyte Polycystic ovary In vitro maturation Endocrinology medicine.anatomical_structure Reproductive Medicine Estrogen Fertilization Oocytes Female Anovulation Polycystic Ovary Syndrome |
| Zdroj: | Fertility and Sterility. 62:353-362 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(16)56891-5 |
| Popis: | Objective To determine the maturational and developmental competence of immature oocytes recovered in situ from anovulatory and ovulatory patients with polycystic ovaries (PCO). Design A newly designed method for recovery of immature oocytes from 2 to 10mm follicles by transvaginal ultrasound or laparoscopy was used to compare the recovery and maturation of oocytes from 9 anovulatory polycystic ovarian syndrome (PCOS) patients and 10 ovulatory patients without polycystic ovaries (PCO) (experiment 1). In a second study (experiment 2), we compared the maturation, fertilization, and development of oocytes recovered from another 10 anovulatory PCOS and 13 ovulatory PCO patients. Two types of culture methods and time intervals for maturation were also examined. Results In experiment 1, a significantly higher number of immature oocytes were recovered from PCOS patients (15.3) compared with non-PCO patients (2.8). Sixty-five percent of oocytes cultured in medium with gonadotropins, estrogen, and fetal calf serum matured to metaphase II by 43 to 47hours, and 81% were mature at 48 to 54hours of culture. Thirty-four percent of the inseminated oocytes fertilized and 56% of the cultured pronuclear oocytes cleaved to eight cells or more. In experiment 2, there was no significant difference between anovulatory PCOS and ovulatory PCO patients in the number of oocytes recovered or their maturation, fertilization, and development. There was no difference between oocytes matured in medium or in coculture with mature granulosa cells, with or without added hCG. However, significantly fewer oocytes were immature and more fertilized when oocytes were inseminated after 34.5 to 35.5hours of maturation than 29.5 to 32.5hours of maturation. A pregnancy and the birth of a normal baby occurred in one of the anovulatory PCOS patient receiving an abbreviated steroid replacement protocol after ET. Conclusion Immature oocyte recovery could be developed as a new method for the treatment of women with infertility due to PCO because the oocytes of these patients retain their maturational and developmental competence. |
| Databáze: | OpenAIRE |
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