Measuring relative utilization of aerobic glycolysis in breast cancer cells by positional isotopic discrimination
| Autor: | Margot P. Cleary, Adrian D. Hegeman, Da Qing Yang, Dana M. Freund, Defeng Wang, Benjamin R E Harris |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Spectrometry Mass Electrospray Ionization Glucose uptake Biophysics Breast Neoplasms Biology Biochemistry Article 03 medical and health sciences Mice Breast cancer Structural Biology Internal medicine Cell Line Tumor Genetics medicine Animals Humans Glycolysis Lactic Acid Molecular Biology Cancer Cell Biology medicine.disease Metastatic breast cancer Mice Inbred C57BL 030104 developmental biology Endocrinology Anaerobic glycolysis Cancer cell Biomarker (medicine) Female |
| Popis: | The ability of cancer cells to produce lactate through aerobic glycolysis is a hallmark of cancer. In this study, we established a positional isotopic labeling and LC-MS-based method that can specifically measure the conversion of glucose to lactate in glycolysis. We show that the rate of aerobic glycolysis is closely correlated with glucose uptake and lactate production in breast cancer cells. We also found that the production of [3-(13) C]lactate is significantly elevated in metastatic breast cancer cells and in early stage metastatic mammary tumors in mice. Our findings may enable the development of a biomarker for the diagnosis of aggressive breast cancer. |
| Databáze: | OpenAIRE |
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