Differentiation-Arrested Rat Fetal Lung in Primary Monolayer Cell Culture
| Autor: | Tanswell Ak, Vreeken E, Joneja Mg, Lindsay J |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Fetus Triiodothyronine Cell division Cell growth Chemistry Insulin medicine.medical_treatment Clinical Biochemistry Gestational age Lamellar granule In vitro Andrology Endocrinology Cell culture In vivo Internal medicine medicine Secretion Epithelial cell maturation Molecular Biology Dexamethasone medicine.drug Epithelial cell differentiation |
| Zdroj: | Experimental Lung Research. 5:37-48 |
| ISSN: | 1521-0499 0190-2148 |
| DOI: | 10.3109/01902148309061503 |
| Popis: | Differentiation-arrested and hormone-depleted monolayer cultures were developed from rat fetal lungs of 18-, 19-, 20-, and 22-days gestation. Incorporation of [3H]-choline into saturated phosphatidylcholine increased, whereas the rate of cell division decreased, with advancing gestational age. Both functions were modified by physiological concentrations of glucocorticoids, thyroid hormones, and insulin. Dexamethasone (0.055-5.5 nM) increased [3H]-choline incorporation into total saturated phosphatidylcholine in immature cultures only, but caused secretion in mature (day-22) cultures. Triiodothyronine (0.055-5.5 nM) increased [3H]-choline incorporation into total and secreted saturated phosphatidylcholine at all gestational ages. Insulin (5-50 microU/ml) inhibition of [3H]-choline incorporation into saturated phosphatidylcholine was evident only in mature cultures. Dexamethasone (0.55 nM), triiodothyronine (5.5 nM), and insulin (50 microU/ml) also had gestation-dependent effects on cell division. |
| Databáze: | OpenAIRE |
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