Mouse Fat-Specific Protein 27 (FSP27) expressed in plant cells localizes to lipid droplets and promotes lipid droplet accumulation and fusion
Autor: | Srikarthika Jambunathan, Alyssa Schami, Satinder K. Gidda, Olga Yurchenko, Vishwajeet Puri, Robert T. Mullen, John M. Dyer, Christopher N. James, Ann Price, Kent D. Chapman, Nathan M. Doner |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Nicotiana tabacum Genetic Vectors Arabidopsis Gene Expression Membrane Fusion 7. Clean energy Biochemistry Mice 03 medical and health sciences Plant Cells Lipid droplet Tobacco Animals Arabidopsis thaliana Diacylglycerol O-Acyltransferase Cloning Molecular Nicotiana 2. Zero hunger 030102 biochemistry & molecular biology biology Effector Chemistry fungi Proteins food and beverages Lipid Droplets General Medicine Lipid Metabolism Plants Genetically Modified biology.organism_classification Plant cell Recombinant Proteins Cell biology Plant Leaves 030104 developmental biology Organelle Size Seeds Ectopic expression |
Zdroj: | Biochimie. 169:41-53 |
ISSN: | 0300-9084 |
Popis: | Fat-Specific Protein 27 (FSP27) belongs to a small group of vertebrate proteins containing a Cell-death Inducing DNA fragmentation factor-α-like Effector (CIDE)-C domain and is involved in lipid droplet (LD) accumulation and energy homeostasis. FSP27 is predominantly expressed in white and brown adipose tissues, as well as liver, and plays a key role in mediating LD-LD fusion. No orthologs have been identified in invertebrates or plants. In this study, we tested the function of mouse FSP27 in stably-transformed Arabidopsis thaliana leaves and seeds, as well as through transient expression in Nicotiana tabacum suspension-cultured cells and N. benthamiana leaves. Confocal microscopic analysis of plant cells revealed that, similar to ectopic expression in mammalian cells, FSP27 produced in plants 1) correctly localized to LDs, 2) accumulated at LD-LD contact sites, and 3) induced an increase in the number and size of LDs and also promoted LD clustering and fusion. Furthermore, FSP27 increased oil content in transgenic A. thaliana seeds. Given that plant oils have uses in human and animal nutrition as well as industrial uses such as biofuels and bioplastics, our results suggest that ectopic expression of FSP27 in plants represents a potential strategy for increasing oil content and energy density in bioenergy or oilseed crops. |
Databáze: | OpenAIRE |
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