Comprehensive Analysis of Differentially Expressed mRNA, lncRNA and circRNA and Their ceRNA Networks in the Longissimus Dorsi Muscle of Two Different Pig Breeds

Autor: Hua Liushuai, Hai Cao, Chen Junfeng, Xing Baosong, Jing Wang, Li Haili, Xu Bin, Bai Xianxiao, Shi Zhihai, Zhang Jiaqing, Ren Qiaoling, Bai Hongjie
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
pig
Swine
Muscle Development
lcsh:Chemistry
0302 clinical medicine
lncRNA
circRNA
lcsh:QH301-705.5
Spectroscopy
Adipogenesis
Cell Differentiation
General Medicine
musculoskeletal system
Computer Science Applications
Cell biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
RNA
Long Noncoding
Biology
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Circular RNA
microRNA
medicine
Animals
RNA
Messenger
Physical and Theoretical Chemistry
skeletal muscle
Muscle
Skeletal
Molecular Biology
Gene
Messenger RNA
Competing endogenous RNA
Gene Expression Profiling
Organic Chemistry
Skeletal muscle
RNA
RNA
Circular
ceRNA
MicroRNAs
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
ceRNA
lncRNA
circRNA
pig
skeletal muscle
Transcriptome
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 5, p 1107 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 5
ISSN: 1422-0067
Popis: Circular RNA (circRNA) and long non-coding RNA (lncRNA) are known to participate in adipogenesis and myogenic differentiation, but their impact on porcine muscle traits is not well understood. We compared their expressional profiles in the longissimus dorsi muscle of Chinese Huainan pigs (HN, the fat type) and Western commercial Duroc ×
(Landrace ×
Yorkshire) (DLY, the thin type) pigs, and 854 mRNAs, 233 lncRNAs, and 66 circRNAs (p <
0.05 and | log2FoldChange | >
1) were found to be differentially expressed. The differentially expressed mRNA and circRNA parental genes were enriched in the Wnt signaling pathway (adipogenesis), the transition between fast and slow fibers (myogenic differentiation), and alanine, aspartate and glutamate metabolism (pork flavor). The potential lncRNAs/circRNAs-miRNAs-mRNAs regulatory networks shared MYOD1, PPARD, miR-423-5p and miR-874, which were associated with skeletal muscle muscular proliferation, differentiation/regeneration and adipogenesis. Taken together, these differentially expressed non-coding RNAs may be involved in the molecular basis of muscle traits, acting as the competing endogenous RNA (ceRNA) for miRNAs.
Databáze: OpenAIRE
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