Evaluation of a co-product of biodiesel production as carbon source in the production of biosurfactant by P. aeruginosa MSIC02
| Autor: | Luciana Rocha Barros Gonçalves, Jessyca Aline da Costa Correia, José Gustavo L Almeida, Vânia Maria Maciel Melo, Juliana Rabelo Sousa, Sueli Rodrigues, Otília Deusdênia L. Pessoa |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Biodiesel
business.industry Organic Chemistry Rhamnolipid Glycerin Biomass Bioengineering Transesterification Biosurfactan Applied Microbiology and Biotechnology Biochemistry Industrial and Manufacturing Engineering Biotechnology chemistry.chemical_compound chemistry Biofuel Biodiesel production Pseudomonas aeruginosa Glycerol Food science business Energy source Engineering (miscellaneous) |
| Zdroj: | Process Biochemistry. 46(9):1831-1839 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2011.06.016 |
| Popis: | This work focuses on the use of a co-product of biodiesel production as carbon and energy source for the production of rhamnolipids by a new strain of Pseudomonas aeruginosa MSIC02. Different carbon sources were compared and the highest rhamnolipid concentration (1269.79 mg/L) was achieved when hydrolyzed glycerin was used. The study of nutritional and environmental conditions allowed an increase in the production of rhamnolipids. Maximum rhamnolipid concentration was attained at 18 g/L of glycerol, 4.0 g/L of NaNO 3 , 62 mM of KH 2 PO 4 , pH 7.0 and 37 °C. Highest product yields on substrate and biomass and productivity were achieved at the same conditions. The biosurfactant showed good emulsifying properties (IE 24 = 65%), being able to form emulsions with mineral and vegetable oils. The NMR spectrum of H 1 and C 13 and mass spectra indicates that the purified product contained two types of rhamnolipids: l -rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (RL1) and l -rhamnosyl l -rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (RL2). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect