Effect of Mast Cell Chymase on the Morphology of Thyroid Cells in vitro
| Autor: | R. De Forteza, K. Banovac, E. Koren |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Cell Survival
Immunology Thyroid Gland Fluorescent Antibody Technique Connective tissue Biology Cell Line Extracellular matrix Chymases Tubulin medicine Animals Humans Immunology and Allergy Mast Cells Cells Cultured Serine Endopeptidases Thyroid General Medicine Chromatography Ion Exchange Mast cell Actins In vitro Rats Interleukin 33 medicine.anatomical_structure Cell culture Microscopy Electron Scanning Endocrine gland |
| Zdroj: | International Archives of Allergy and Immunology. 99:133-140 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000236347 |
| Popis: | We studied the effect of mast cell chymase on the thyroid cells in culture. Rat serosal mast cells, similar functionally to connective tissue mast cells, were obtained after lavage of the peritoneal cavity and lyzed by freezing. The resulting lysate was used as crude enzyme preparation. Mast cell chymase was purified from the crude preparation by anion exchange chromatography. Crude and purified chymase incubated with thyroid cells induced cellular retraction, the appearance of long processes and gradual cell detachment from the substratum. The effect of the enzyme was not cytotoxic. The immunofluorescence studies of thyroid cells showed a decreased amount of polymerized actin and tubulin after incubation with chymase. Neutral protease inhibitor abolished the effect of crude and purified chymase on thyroid cell morphology. The above findings suggest that mast cell chymase may have a function in the control of cell morphology and cell-matrix interaction. |
| Databáze: | OpenAIRE |
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