Incorporation by chemical synthesis and characterization of deoxyribosylformylamine into DNA
| Autor: | Anne-Marie Duplaa, Robert Teoule, Jacques Ulrich, A. Guy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
DNA Replication
Phosphoramidite Guanine Base Sequence Stereochemistry Oligonucleotide DNA damage Deoxyribose Molecular Sequence Data DNA-Directed DNA Polymerase Biology Chemical synthesis In vitro Mass Spectrometry chemistry.chemical_compound Residue (chemistry) Organophosphorus Compounds chemistry Biochemistry Oligodeoxyribonucleotides Genetics Taq Polymerase DNA DNA Damage |
| Popis: | 2-deoxyribosylformylamine is a major oxidative DNA damage type which occurs upon the action of ionizing radiation on DNA. The protected 2-deoxyribosylformylamine phosphoramidite was synthesized and used in conjunction with previously reported alkali labile base protected phosphoramidites ('PAC phosphoramidites') for the preparation of oligodeoxyribonucleotides containing this lesion. Final deprotection of the oligonucleotides was performed under mild alkaline conditions to preserve the integrity of the fragile defect. The presence of formylamino deoxyribosyl residue was confirmed by FAB mass spectrometry sequencing. Oligonucleotides bearing deoxyribosyl formylamine were used as templates for studying in vitro replication. They direct the insertion of guanine or induce a deletion opposite the lesion. |
| Databáze: | OpenAIRE |
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