Expression of nitric oxide synthases in rat odontoblasts and the role of nitric oxide in odontoblastic differentiation of rat dental papilla cells

Autor:	Jun Kang, Hongwen He, Yu Kong, Haoling Chen, Fang Huang, Wenguo Fan, Tong Yan
Rok vydání:	2021
Předmět:	Odontoblasts Cellular differentiation Cell Differentiation Cell Biology Biology Nitric Oxide Endothelial NOS DMP1 Rats Nitric oxide Cell biology Extracellular matrix stomatognathic diseases chemistry.chemical_compound Odontoblast stomatognathic system chemistry Dentin sialophosphoprotein Animals Nitric Oxide Synthase Dental papilla Dental Papilla Cells Cultured Dental Pulp Developmental Biology
Zdroj:	Development, Growth & Differentiation. 63:354-371
ISSN:	1440-169X 0012-1592
Popis:	As precursor cells of odontoblasts, dental papilla cells (DPCs) form the dentin-pulp complex during tooth development. Nitric oxide (NO) regulates the functions of multiple cells and organ tissues, including stem cell differentiation and bone formation. In this paper, we explored the involvement of NO in odontoblastic differentiation. We verified the expression of NO synthase (NOS) in rat odontoblasts by nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining and immunohistochemistry in vivo. The expression of all three NOS isoforms in rat DPCs was confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunofluorescence, and western blotting in vitro. The expression of neuronal NOS and endothelial NOS were upregulated during the odontoblastic differentiation of DPCs. Inhibition of NOS function by NOS inhibitor, L-NG -monomethyl arginine (L-NMMA), resulted in reduced formation of mineralized nodules and expression of dentin sialophosphoprotein (DSPP) and dentin matrix protein (DMP1) during DPC differentiation. The NO donor S-nitroso-N-acetylpenicillamine (SNAP, 0.1, 1, 10, and 100 μM) promoted the viability of DPCs. Extracellular matrix mineralization and odontogenic markers expression were elevated by SNAP at low concentrations (0.1, 1, and 10 μM) and suppressed at high concentration (100 μM). Blocking the generation of cyclic guanosine monophosphate (cGMP) with 1H-(1,2,4)oxadiazolo-(4,3-a)quinoxalin-1-one (ODQ) abolished the positive influence of SNAP on the odontoblastic differentiation of DPCs. These findings demonstrate that NO regulates the odontoblastic differentiation of DPCs, and thereby influencing dentin formation and tooth development.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cada1d7c94230522ddce1ddbbf4e1484 https://doi.org/10.1111/dgd.12745 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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