Changes of Tight Junction Protein Claudins in Small Intestine and Kidney Tissues of Mice Fed a DDC Diet
| Autor: | Masaya Takeuchi, Masaki Murata, Yukie Abiko, Takashi Kojima, Michio Mori, Mitsuhiro Tsujiwaki, Norimasa Sawada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Kidney
Pathology medicine.medical_specialty kidney Tight junction Short Communication tight junction Crypt Biology Toxicology Subcellular localization urologic and male genital diseases Molecular biology digestive system Small intestine digestive system diseases Pathology and Forensic Medicine medicine.anatomical_structure DDC diet medicine Immunohistochemistry Claudin claudins small intestine Barrier function |
| Zdroj: | Journal of Toxicologic Pathology |
| ISSN: | 1881-915X 0914-9198 |
| Popis: | DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine)-fed mice are widely used as a model for cholestatic liver disease. We examined the expression of tight junction protein claudin subspecies by immunofluorescent histochemistry in small intestine and kidney tissues of mice fed a DDC diet for 12 weeks. In the small intestine, decreases in claudin-3, claudin-7 and claudin-15 were observed in villous epithelial cells corresponding to the severity of histological changes while leaving the abundance of these claudin subspecies unchanged in crypt cells. Nevertheless, the proliferative activity of intestinal crypt cells measured by immunohistochemistry for Ki-67 decreased in the mice fed the DDC diet compared with that of control mice. These results suggest the possibility that DDC feeding affects the barrier function of villous epithelial cells and thus inhibits the proliferative activity of crypt epithelial cells. On the other hand, in the kidney, remarkable changes were found in the subcellular localization of claudin subspecies in a segment-specific manner, although histological changes of renal epithelial cells were quite minimal. These results indicate that immunohistochemistry for claudin subspecies can serve as a useful tool for detecting minute functional alterations of intestinal and renal epithelial cells. |
| Databáze: | OpenAIRE |
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