Mouse Blastocysts Pulse Labeled with 14C-Glucosamine: Incorporation and Ultrastructural Analysis
| Autor: | Roger A. Acey, Danica Dabich, Linda D. Hazlett |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Time Factors
Golgi Apparatus Biology Microtubules Mice chemistry.chemical_compound symbols.namesake Glucosamine medicine Animals Carbon Radioisotopes Blastocyst Cell Nucleus Inclusion Bodies Pulse (signal processing) Blastocoel Trophoblast Embryo Cell Biology General Medicine Golgi apparatus Lipid Metabolism Molecular biology Mitochondria Cell biology Cell nucleus medicine.anatomical_structure Reproductive Medicine chemistry embryonic structures symbols Female |
| Zdroj: | Biology of Reproduction. 16:564-570 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod16.5.564 |
| Popis: | In vitro pulse labeling of early mouse blastocysts (90 h p.c.) with 4C-glucosamine yielded linear time profiles for both total uptake of radioactivity and incorporation into acid precipitable material.Radioautographic finestructuralanalysis of pulse labeled embryos demonstrated distinct intra- and inter-cellularsitesof labeling.Among these are precipitatedfibrillarmaterial in the blastocoel,trophoblast cellsurfaces,lipiddroplets,microtubules, Golgi complex, mitochondria, the nuclear envelope and nucleolarassociatedchromatin regions. |
| Databáze: | OpenAIRE |
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