Measurement of ascorbic acid in human plasma and urine by high-performance liquid chromatography results in healthy subjects and patients with idiopathic calcium urolithiasis
| Autor: | M. Manoharan, P.O. Schwille |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Quality Control Urinary system Ascorbic Acid Urine Sensitivity and Specificity High-performance liquid chromatography chemistry.chemical_compound Reference Values Blood plasma Humans Phosphoric Acids Chromatography High Pressure Liquid Aged Chromatography Chemistry hemic and immune systems General Chemistry Reversed-phase chromatography Middle Aged Ascorbic acid eye diseases Calcium Female Urinary Calculi Dehydroascorbic acid Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 654:134-139 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(94)00008-5 |
| Popis: | A simple, reliable high-performance liquid chromatographic method was developed to measure ascorbic acid (ASC), with ultraviolet detection (250 nm), in human plasma and urine. Immediately following blood withdrawal, the heparinized plasma samples were deproteinized with 10% m-phosphoric acid, while the freshly voided urine samples were diluted with m-phosphoric acid. ASC was separated on a reversed-phase column by elution with 0.1 M KH2PO4 adjusted to pH 2.35. In urine, after reduction of dehydroascorbic acid to ASC, total ASC was measured using the same mobile phase. The method was sensitive down to 0.1 and 0.4 mg ASC per litre of urine and plasma, respectively. In patients with idiopathic calcium urolithiasis, both plasma and urinary ASC were within the range observed in age-matched controls. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|