Effects of Adenine Methylation on the Structure and Thermodynamic Stability of a DNA Minidumbbell
| Autor: | Hung Kay Lee, Pei Guo, Sik Lok Lam, Liqi Wan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Base pair Hoogsteen base pair 010402 general chemistry 01 natural sciences Article Catalysis Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY A-DNA N1-methyladenine N6-methyladenine Nucleotide Motifs Physical and Theoretical Chemistry Base Pairing Nuclear Magnetic Resonance Biomolecular Molecular Biology lcsh:QH301-705.5 Spectroscopy nuclear magnetic resonance spectroscopy DNA minidumbbell Adenine methylation DNA methylation Molecular Structure Chemistry Adenine Organic Chemistry Hydrogen Bonding DNA General Medicine Nuclear magnetic resonance spectroscopy 0104 chemical sciences Computer Science Applications 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Biophysics Nucleic Acid Conformation Thermodynamics Chemical stability |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 7 International Journal of Molecular Sciences, Vol 22, Iss 3633, p 3633 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22073633 |
| Popis: | DNA methylation is a prevalent regulatory modification in prokaryotes and eukaryotes. N1-methyladenine (m1A) and N6-methyladenine (m6A) have been found to be capable of altering DNA structures via disturbing Watson–Crick base pairing. However, little has been known about their influences on non-B DNA structures, which are associated with genetic instabilities. In this work, we investigated the effects of m1A and m6A on both the structure and thermodynamic stability of a newly reported DNA minidumbbell formed by two TTTA tetranucleotide repeats. As revealed by the results of nuclear magnetic resonance spectroscopic studies, both m1A and m6A favored the formation of a T·m1A and T·m6A Hoogsteen base pair, respectively. More intriguingly, the m1A and m6A modifications brought about stabilization and destabilization effects on the DNA minidumbbell, respectively. This work provides new biophysical insights into the effects of adenine methylation on the structure and thermodynamic stability of DNA. |
| Databáze: | OpenAIRE |
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