Cedrus deodara: In vitro antileishmanial efficacy & immumomodulatory activity
| Autor: | Shyam Narayan, Shashi Nath Pandey, Meenakshi Thakur, Pulok K. Mukherjee, Shiv Bahadur, Chandreshwar Prasad Thakur, Dipendra Kumar Mitra, Ajit K. Thakur |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Arginase - Cedrus deodara - linalool - reverse-phase high-performance liquid chromatography - visceral leishmaniasis Cedrus deodara linalool 030231 tropical medicine 030106 microbiology Leishmania donovani Ethyl acetate lcsh:Medicine reverse-phase high-performance liquid chromatography General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Linalool medicine Bioassay visceral leishmaniasis biology Traditional medicine Arginase Chemistry lcsh:R Leishmaniasis General Medicine medicine.disease Rotary evaporator biology.organism_classification Visceral leishmaniasis Original Article |
| Zdroj: | Indian Journal of Medical Research, Vol 146, Iss 6, Pp 780-787 (2017) The Indian Journal of Medical Research |
| ISSN: | 0971-5916 |
| Popis: | Background & objectives: The existing antileishmanial drugs for complete cure of visceral leishmaniasis (kala-azar) are limited. The available drugs are either toxic or less effective leading to disease relapse or conversion to post-kala-azar dermal leishmaniasis. Several herbal extracts have been shown to have antileishmanial activity, but a herbal drug may not always be safe. In the present study, the extract of Cedrus deodara leaves has been standardized and tested for immunomodulatory antileishmanial activities. Methods: The extracts of C. deodara leaves with different solvents such as benzene, chloroform, ethyl acetate and methanol were made by soxhlation process. Solvents were removed under reduced pressure and temperature using rotary evaporator. The antileishmanial bioassay test was performed with in vitro maintained parasites. Immunomodulatory activity of different extracts was tested by flow cytometry. Standardization of the effective fraction was performed with Linalool as a marker compound through reverse-phase high-performance liquid chromatography. >Results: The extract with the use of benzene solvent showed strong antileishmanial activities within a dose 25-200 μg/ml culture with non-significant haemolytic activities and significant immunomodulant activities against the host cells. Linalool was found to be 1.29 per cent in the effective extract of C. deodara. >Interpretation & conclusions: The antileishmanial activity of C. deodara, as assessed by bioassay testing onLeishmania donovani parasites and immunomodulatory effect of benzene extract of leaves on host cells indicated that it might be a potential new safe therapeutic target to cure the visceral leishmaniasis. |
| Databáze: | OpenAIRE |
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