Mycophenolic Acid Inhibits Syncytium Formation Accompanied by Reduction of gp120 Expression
| Autor: | Hideaki Ui, Yuichi Yamaguchi, Atsushi Takahashi, Rokuro Masuma, Satoshi Omura, Satoshi Asanuma, Harumi Chiba, Haruo Tanaka |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
viruses
Blotting Western education Gene Expression HIV Envelope Protein gp120 Biology Antiviral Agents Giant Cells Mycophenolic acid Genes Reporter immune system diseases Drug Discovery medicine Humans Enzyme Inhibitors Pharmacology Syncytium Chemistry virus diseases General Medicine Mycophenolic Acid biochemical phenomena metabolism and nutrition Molecular biology HIV-1 HeLa Cells medicine.drug |
| Zdroj: | The Journal of Antibiotics. 58:514-518 |
| ISSN: | 1881-1469 0021-8820 |
| DOI: | 10.1038/ja.2005.69 |
| Popis: | Mycophenolic acid (MPA) was identified as an inhibitor of syncytium formation during the screening of human immunodeficiency virus (HIV) entry inhibitors. MPA is a well-known inhibitor of inosine monophosphate dehydrogenase and anti-HIV activity has been reported in vitro and in vivo. MPA inhibited syncytium formation in T cell-tropic and macrophage-tropic systems with IC50 values of 0.1 and 0.5 microM, respectively. The reduction of HIV gp120 expression by MPA (1.0 microM) was observed by use of Western blot analysis. Furthermore, the addition of guanosine restored both syncytium formation and gp120 expression in the presence of MPA. These results suggest that MPA inhibits not only reverse transcription by depletion of a substrate, GTP, as has been reported, but also syncytium formation through a predominant reduction in the amount of gp120 that is vigorously expressed in the above transformed cells and may be in HIV-infected cells. |
| Databáze: | OpenAIRE |
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