Imaging of intratumoral inflammation during oncolytic virotherapy of tumors by 19F-magnetic resonance imaging (MRI)

Autor: Stephanie Weibel, Yu-Xiang Ye, Volker Sturm, Michael Hess, Thomas Christian Basse-Luesebrink, Thomas Kampf, Carolin Seubert, Johanna Langbein-Laugwitz, Aladar A. Szalay, Peter M. Jakob, Elisabeth Hofmann, Ivaylo Gentschev
Rok vydání: 2012
Předmět:
Pathology
Medical Physics
Mouse
Cancer Treatment
lcsh:Medicine
Diagnostic Radiology
Mice
Neoplasms
Nanotechnology
lcsh:Science
Oncolytic Virotherapy
Fluorocarbons
Multidisciplinary
medicine.diagnostic_test
Chemistry
Animal Models
Magnetic Resonance Imaging
Oncolytic Viruses
Oncology
Medicine
Female
Oncology Agents
Immunotherapy
medicine.symptom
Radiology
Preclinical imaging
Research Article
medicine.medical_specialty
Genetic Vectors
Transplantation
Heterologous
Antigens
Differentiation
Myelomonocytic
Inflammation
Vaccinia virus
Microbiology
Virus
Immune system
Spatio-Temporal Analysis
Model Organisms
Antigen
Antigens
CD
Cell Line
Tumor
Virology
medicine
ddc:572
Cancer Detection and Diagnosis
Animals
Humans
Biology
Macrophages
lcsh:R
Magnetic resonance imaging
Viral Vaccines
Oncolytic virus
Transplantation
Disease Models
Animal
Viruses and Cancer
Nanoparticles
lcsh:Q
Zdroj: PLoS ONE
PLoS ONE, Vol 8, Iss 2, p e56317 (2013)
ISSN: 1932-6203
Popis: Background Oncolytic virotherapy of tumors is an up-coming, promising therapeutic modality of cancer therapy. Unfortunately, non-invasive techniques to evaluate the inflammatory host response to treatment are rare. Here, we evaluate \(^{19}\)F magnetic resonance imaging (MRI) which enables the non-invasive visualization of inflammatory processes in pathological conditions by the use of perfluorocarbon nanoemulsions (PFC) for monitoring of oncolytic virotherapy. Methodology/Principal Findings The Vaccinia virus strain GLV-1h68 was used as an oncolytic agent for the treatment of different tumor models. Systemic application of PFC emulsions followed by \(^1H\)/\(^{19}\)F MRI of mock-infected and GLV-1h68-infected tumor-bearing mice revealed a significant accumulation of the \(^{19}\)F signal in the tumor rim of virus-treated mice. Histological examination of tumors confirmed a similar spatial distribution of the \(^{19}\)F signal hot spots and \(CD68^+\)-macrophages. Thereby, the \(CD68^+\)-macrophages encapsulate the GFP-positive viral infection foci. In multiple tumor models, we specifically visualized early inflammatory cell recruitment in Vaccinia virus colonized tumors. Furthermore, we documented that the \(^{19}\)F signal correlated with the extent of viral spreading within tumors. Conclusions/Significance These results suggest \(^{19}\)F MRI as a non-invasive methodology to document the tumor-associated host immune response as well as the extent of intratumoral viral replication. Thus, \(^{19}\)F MRI represents a new platform to non-invasively investigate the role of the host immune response for therapeutic outcome of oncolytic virotherapy and individual patient response.
Databáze: OpenAIRE
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