Experimental infections with Mycoplasma agalactiae identify key factors involved in host-colonization
| Autor: | Marie-Claude Hygonenq, Dominique Bergonier, Eveline Sagné, Patricia Ronsin, Eric Baranowski, Xavier Berthelot, Christine Citti |
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| Přispěvatelé: | Citti, Christine, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Ecole Nationale Vétérinaire de Toulouse (ENVT), INRA (Animal Health Division, AAP), ENVT |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
infection expérimentale
Mycoplasma agalactiae [SDV]Life Sciences [q-bio] ved/biology.organism_classification_rank.species Veterinary Microbiology lcsh:Medicine Pathogenesis medicine.disease_cause Pathology and Laboratory Medicine Medicine and Health Sciences Axenic lcsh:Science Pathogen Infectivity 0303 health sciences Multidisciplinary biology Veterinary Bacteriology Phenotype 3. Good health Bacterial Pathogens Veterinary Diseases Medical Microbiology Host-Pathogen Interactions Female Veterinary Pathology Research Article Sheep Diseases Locus (genetics) Microbiology 03 medical and health sciences Bacterial Proteins medicine Animals colonisation bactérienne Mycoplasma Infections Microbial Pathogens mycoplasma agalactiae 030304 developmental biology Gram Positive Sheep 030306 microbiology ved/biology lcsh:R Biology and Life Sciences Bacteriology Mycoplasma biology.organism_classification Genetic Loci Mutation Transposon mutagenesis lcsh:Q Veterinary Science |
| Zdroj: | Plos One 4 (9), . (2014) PLoS ONE PLoS ONE, Public Library of Science, 2014, 9 (4), ⟨10.1371/journal.pone.0093970⟩ PLoS ONE, Vol 9, Iss 4, p e93970 (2014) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0093970⟩ |
| Popis: | International audience; Mechanisms underlying pathogenic processes in mycoplasma infections are poorly understood, mainly because of limited sequence similarities with classical, bacterial virulence factors. Recently, large-scale transposon mutagenesis in the ruminant pathogen Mycoplasma agalactiae identified the NIF locus, including nifS and nifU, as essential for mycoplasma growth in cell culture, while dispensable in axenic media. To evaluate the importance of this locus in vivo, the infectivity of two knock-out mutants was tested upon experimental infection in the natural host. In this model, the parental PG2 strain was able to establish a systemic infection in lactating ewes, colonizing various body sites such as lymph nodes and the mammary gland, even when inoculated at low doses. In these PG2-infected ewes, we observed over the course of infection (i) the development of a specific antibody response and (ii) dynamic changes in expression of M. agalactiae surface variable proteins (Vpma), with multiple Vpma profiles co-existing in the same animal. In contrast and despite a sensitive model, none of the knock-out mutants were able to survive and colonize the host. The extreme avirulent phenotype of the two mutants was further supported by the absence of an IgG response in inoculated animals. The exact role of the NIF locus remains to be elucidated but these data demonstrate that it plays a key role in the infectious process of M. agalactiae and most likely of other pathogenic mycoplasma species as many carry closely related |
| Databáze: | OpenAIRE |
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