Molecular characterization of Trypanosoma cruzi and Leishmania spp. coinfection in mammals of Venezuelan coendemic areas
Autor: | Mercedes Viettri, Leidi Herrera, Elizabeth Ferrer, María Lares, M.D Feliciangeli, Daisy Lozano-Arias, J Reyes, Tony Chacón, Roberto García-Alzate, Cruz Manuel Aguilar, Antonio Morocoima |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Chagas disease Molecular epidemiology biology 030106 microbiology 030231 tropical medicine Leishmaniasis General Medicine medicine.disease biology.organism_classification Leishmania Virology lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Infectious Diseases Canis parasitic diseases Coinfection medicine Parasitology lcsh:RC109-216 Trypanosoma cruzi Trypanosomiasis coinfection leishmania mammals molecular characterization reservoirs trypanosoma cruzi |
Zdroj: | Journal of Vector Borne Diseases, Vol 56, Iss 3, Pp 252-262 (2019) |
ISSN: | 0972-9062 |
Popis: | Background & objectives: Trypanosoma cruzi and Leishmania spp. are protozoans that cause American trypanosomiasis and leishmaniasis, respectively. In endemic foci where both diseases coincide, coinfection can occur. The objective of this work was the characterization of the parasites involved in coinfection in several endemic areas of Venezuela. Methods: Molecular characterization was done in 30 samples of several species of mammals (Didelphis marsupialis, Equus mulus, Rattus rattus, Canis familiaris, Felis catus, and Sciurus granatensis) from the states of Anzoategui, Cojedes and Capital District diagnosed with T. cruzi and Leishmania spp. coinfections. For the typing of T. cruzi DTUs, the markers of miniexon, 24Sa rDNA, 18Sa rDNA, and hsp60-PCR-RFLP (EcoRV) were used. Infection by Leishmania spp. was characterized by miniexon multiplex PCR for complexes of Leishmania and ITS1-PCR-RFLP (HaeIII, HhaI, and RsaI) for the identification of the species. Results: The T. cruzi TcI was present in 100% of the coinfected mammals, which included 76.7% of triple infection by T. cruzi TcI-complex–L. (L) mexicana–L. infantum/chagasi, 13.3% of double infection by T. cruzi TcI-L. mexicana and 10% of double infection by T. cruzi Tcl—L. infantum/chagasi. Interpretation & conclusion: These results suggest that the double or triple infection is a phenomenon existing in almost all the coendemics areas and mammals studied, which might influence the mechanisms of adaptation and pathogenicity of these parasites. |
Databáze: | OpenAIRE |
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