Selective cell propagation via micropatterning of a thermally-activated hydrogel
| Autor: | Kerryn Matthews, Jeffrey C.Y. Chiu, Simon P. Duffy, Hongshen Ma, Jeong Hyun Lee, Joyce A. Teodoro |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Kinetics Cell Biomedical Engineering Bioengineering 02 engineering and technology CHO Cells Biochemistry law.invention Flow cytometry 03 medical and health sciences Cricetulus law Lab-On-A-Chip Devices medicine Animals 030304 developmental biology 0303 health sciences medicine.diagnostic_test Chinese hamster ovary cell Far-infrared laser Temperature Hydrogels General Chemistry 021001 nanoscience & nanotechnology Laser Flow Cytometry medicine.anatomical_structure Self-healing hydrogels Biophysics Single-Cell Analysis 0210 nano-technology Micropatterning |
| Zdroj: | Lab on a chip. 20(9) |
| ISSN: | 1473-0189 |
| Popis: | The ability to selectively propagate specific cells is fundamentally important to the development of clonal cell populations. Current methods rely on techniques such as limiting dilution, colony picking, and flow cytometry to transfer single cells into single wells, resulting in workflows that are low-throughput, slowed by propagation kinetics, and susceptible to contamination. Here, we developed a method, called selective laser gelation (SLG), to micropattern hydrogels in cell culture media in order to encapsulate specific cells to selectively arrest their growth. This process relies on the inverse gelation of methylcellulose, which forms a hydrogel when heated rather than cooled. Local heating using an infrared laser enables hydrogel micropatterning, while phase transition hysteresis retains the hydrogel after laser excitation. As a demonstration, we used this approach to selectively propagate transgenic CHO cells with increased antibody productivity. More generally, hydrogel micropatterning provides a simple and non-contact method for selective propagation of cells based on features identified by imaging. |
| Databáze: | OpenAIRE |
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