Treatment of Streptozotocin-Induced Diabetes Mellitus in Rats by Transplantation of Islet Cells from Two Major Histocompatibility Complex Disparate Rats in Combination with Intra Bone Marrow Injection of Allogeneic Bone Marrow Cells
| Autor: | A-Hon Kwon, Yasuo Kamiyama, Keizo Takada, Hiroko Hisha, Susumu Baba, Junichi Fukui, Yusuke Ueda, Susumu Ikehara, Mitsuru Taira, Muneo Inaba |
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| Rok vydání: | 2005 |
| Předmět: |
Blood Glucose
medicine.medical_specialty Time Factors Islets of Langerhans Transplantation Transplantation Chimera Streptozocin Diabetes Mellitus Experimental Islets of Langerhans Antigen Internal medicine medicine Animals Transplantation Homologous Antigens Bone Marrow Transplantation Transplantation Glucose tolerance test medicine.diagnostic_test business.industry Pancreatic islets Graft Survival Glucose Tolerance Test Streptozotocin Tissue Donors Rats Histocompatibility surgical procedures operative Endocrinology medicine.anatomical_structure Bone marrow Lymphocyte Culture Test Mixed business medicine.drug |
| Zdroj: | Transplantation. 79:680-687 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/01.tp.0000155500.17348.94 |
| Popis: | Background. We have established a new method for the transplantation of allogeneic pancreatic islets obtained from two different rat strains in combination with a newly developed bone marrow transplantation (BMT) method in which bone marrow cells (BMCs) are directly injected into the bone marrow cavity (intra bone marrow BMT [IBM-BMT] ). Methods. Streptozotocin-induced diabetic Brown Norway (BN: RT1A n ) rats were injected with fludarabine, irradiated with 5.0 GyX2, and BMCs from two allogeneic rat strains, Fischer 344 (F344: RT1A 1 ) and PVG (PVG: RT1A c ), were then directly injected into the bone marrow cavity (IBM-BMT). Simultaneously, approximately 600 pancreatic islets (PIs) from F344 and PVG rats were mixed and transplanted into the liver by way of the portal vein. Results. All the recipients thus treated showed normoglycemia 30 days after the treatment. Hematolymphoid cells were completely reconstituted with the two donor-type cells, and immunologic tolerance to F344 and PVG major histocompatibility complex (MHC) determinants were induced. Conclusions. The transplantation of PIs from two MHC-disparate donors was completely achieved in combination with IBM-BMT, resulting in the improvement of blood glucose levels and the amelioration of diabetes mellitus. |
| Databáze: | OpenAIRE |
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