Fail-Safe Transcriptional Termination for Protein-Coding Genes in S. cerevisiae
| Autor: | Junya Kawauchi, Nick J. Proudfoot, Hannah E. Mischo, Ana G. Rondón |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Ribonuclease III
RNA Helicases/genetics Transcription Genetic RNA Stability RNA-binding protein RNA polymerase II Binding Sites/genetics Terminator Regions Genetic/physiology Transcription (biology) RNA Stability/physiology DNA/metabolism Exoribonucleases/genetics 3' Flanking Region Phosphorylation Polymerase Saccharomyces cerevisiae/physiology Terminator Regions Genetic Genetics Acyltransferases/genetics Plasmids/genetics 0303 health sciences Phosphorylation/physiology 030302 biochemistry & molecular biology Nuclear Proteins RNA-Binding Proteins Protein Binding/physiology Cell biology Saccharomyces cerevisiae Proteins/genetics RNA Polymerase II 3' Flanking Region/physiology RNA Helicases Plasmids Protein Binding Saccharomyces cerevisiae Proteins Mutation/genetics PROTEINS Termination factor Ribonuclease III/physiology Saccharomyces cerevisiae Biology Article 03 medical and health sciences RNA Messenger Molecular Biology Gene 030304 developmental biology Messenger RNA Binding Sites DNA Helicases/genetics DNA Helicases DNA Cell Biology RNA Messenger/biosynthesis RNA-Binding Proteins/genetics Nuclear Proteins/genetics Antitermination Exoribonucleases Mutation biology.protein RNA Transcription Genetic/physiology RNA Polymerase II/metabolism Acyltransferases |
| Zdroj: | Molecular Cell Rondón, A G, Mischo, H E, Kawauchi, J & Proudfoot, N J 2009, ' Fail-safe transcriptional termination for protein-coding genes in S. cerevisiae ', MOLECULAR CELL, vol. 36, no. 1, pp. 88-98 . https://doi.org/10.1016/j.molcel.2009.07.028 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2009.07.028 |
| Popis: | Summary Transcription termination of RNA polymerase II (Pol II) on protein-coding genes in S. cerevisiae relies on pA site recognition by 3′ end processing factors. Here we demonstrate the existence of two alternative termination mechanisms that rescue polymerases failing to disengage from the template at pA sites. One of these fail-safe mechanisms is mediated by the NRD complex, similar to termination of short noncoding genes. The other termination mechanism is mediated by Rnt1 cleavage of the nascent transcript. Both fail-safe termination mechanisms trigger degradation of readthrough transcripts by the exosome. However, Rnt1-mediated termination can also enhance the usage of weak pA signals and thereby generate functional mRNA. We propose that these alternative Pol II termination pathways serve the dual function of avoiding transcription interference and promoting rapid removal of aberrant transcripts. |
| Databáze: | OpenAIRE |
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