Preparation of europium-labeled derivatives of cortisol for time-resolved fluoroimmunoassays
| Autor: | Heikki Mikola, Päivi Miettinen |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pharmacology
Chromatography Time Factors Europium chelate Hydrocortisone Molecular Structure Organic Chemistry Clinical Biochemistry Fluoroimmunoassay chemistry.chemical_element Oxime Biochemistry chemistry.chemical_compound Endocrinology chemistry Thioether Europium medicine Molecular Biology hormones hormone substitutes and hormone antagonists Glucocorticoid medicine.drug |
| Zdroj: | Steroids. 56(1) |
| ISSN: | 0039-128X |
| Popis: | Cortisol 3-(O-carboxymethyl)oxime, 6- and 21-hemisuccinoxycortisol, and cortisol 7-carboxyethyl thioether were synthesized. These carboxyl derivatives were labeled using a described general labeling method with a europium chelate. The labeled steroids were tested in a competitive time-resolved fluoroimmunoassay using antibodies raised against cortisol. Only the site-homologous antigen-antibody pairs underwent immunoreaction and gave satisfactory calibration curves. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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