Reconstruction of Bacillus thuringiensis ssp. israelensis Cry11A Endotoxin from Fragments Corresponding to Its N- and C-Moieties Restores Its Original Biological Activity
| Autor: | L. I. Kostina, A. L. Mikhailova, Zalunin Ia, Galina G. Chestukhina, L. A. Ganushkina, L. P. Revina |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Proteases
Mosquito Control animal structures Bacillus thuringiensis Aedes aegypti Biochemistry Microbiology Bacterial Proteins parasitic diseases Animals Anopheles stephensi Serine protease biology Hydrolysis fungi Subtilisin Biological activity General Medicine biology.organism_classification Endotoxins Intestines Lepidoptera Culicidae Larva biology.protein Thermitase Peptide Hydrolases |
| Zdroj: | Biochemistry (Moscow). 69:181-187 |
| ISSN: | 0006-2979 |
| Popis: | Subtilisin hydrolyzes Cry11A endotoxin (of 70 kD) produced by Bacillus thuringiensis ssp. israelensis to fragments of 33- and 36-kD, which correspond to N- and C-terminal halves of the endotoxin molecule. Thermitase (a serine protease from Thermoactinomyces vulgaris) and insect gut proteases from Diptera and Lepidoptera exhibit the same hydrolytic effect on Cry11A. Hydrolyzates maintain high toxicity with respect to larvae of Aedes aegypti, Anopheles stephensi, and Culex pipiens. The 33- and 36-kD Cry11A endotoxin components purified by ion-exchange chromatography from the subtilisin hydrolyzate were inactive; however, equimolar mixture of these proteins exhibited almost the same activity as the initial hydrolyzate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|