Immunobiotic lactobacilli improve resistance of respiratory epithelial cells to sars-cov-2 infection
Autor: | Rafael R. C. Cuadrat, Leonardo Albarracin, Maria Guadalupe Vizoso-Pinto, Jacinto Sacur, Bruno G. N. Andrade, Julio Villena, Mikado Tomokiyo, Haruki Kitazawa, Aminul Islam, J. C. Valdez |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Microbiology (medical)
Lactiplantibacillus plantarum viruses coronavirus Context (language use) CORONAVIRUS SARS-COV-2 Biology LACTIPLANTIBACILLUS PLANTARUM medicine.disease_cause IMMUNOBIOTICS Article Virus Microbiology purl.org/becyt/ford/1 [https] Immune system medicine Immunology and Allergy CXCL10 Interleukin 8 TLR3 purl.org/becyt/ford/1.6 [https] Molecular Biology Coronavirus Innate immune system General Immunology and Microbiology SARS-CoV-2 virus diseases COVID-19 immunobiotics PROBIOTICS Infectious Diseases probiotics RESPIRATORY EPITHELIAL CELLS Medicine Technology Platforms respiratory epithelial cells |
Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Pathogens Volume 10 Issue 9 Pathogens, Vol 10, Iss 1197, p 1197 (2021) |
Popis: | Previously, we reported that immunomodulatory lactobacilli, nasally administered, benefi-cially regulated the lung antiviral innate immune response induced by Toll-like receptor 3 (TLR3) activation and improved protection against the respiratory pathogens, influenza virus and respiratory syncytial virus in mice. Here, we assessed the immunomodulatory effects of viable and non-viable Lactiplantibacillus plantarum strains in human respiratory epithelial cells (Calu-3 cells) and the capacity of these immunobiotic lactobacilli to reduce their susceptibility to the acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Immunobiotic L. plantarum MPL16 and CRL1506 differentially modulated IFN-β, IL-6, CXCL8, CCL5 and CXCL10 production and IFNAR2, DDX58, Mx1 and OAS1 expression in Calu-3 cells stimulated with the TLR3 agonist poly(I:C). Furthermore, the MPL16 and CRL1506 strains increased the resistance of Calu-3 cells to the challenge with SARS-CoV-2. L. plantarum MPL16 induced these beneficial effects more efficiently than the CRL1506 strain. Of note, neither non-viable MPL16 and CRL1506 strains nor the non-immunomodulatory strains L. plantarum CRL1905 and MPL18 could modify the resistance of Calu-3 cells to SARS-CoV-2 infection or the immune response to poly(I:C) challenge. To date, the potential beneficial effects of immunomodulatory probiotics on SARS-CoV-2 infection and COVID-19 outcome have been extrapolated from studies carried out in the context of other viral pathogens. To the best of our knowledge, this is the first demonstration of the ability of immunomodulatory lactobacilli to positively influence the replication of the new coronavirus. Further mechanistic studies and in vivo experiments in animal models of SARS-CoV-2 infection are necessary to identify specific strains of beneficial immunobiotic lactobacilli like L. plantarum MPL16 or CRL1506 for the prevention or treatment of the COVID-19. Fil: Islam, Md Aminul. Tohoku University; Japón Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Tomokiyo, Mikado. Tohoku University; Japón Fil: Valdéz, Juan Carlos. Universidad Nacional de Tucumán; Argentina Fil: Sacur, Jacinto Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Andrade, Bruno G. N.. No especifíca; Fil: Cuadrat, Rafael R. C.. No especifíca; Fil: Kitazawa, Haruki. Tohoku University; Japón Fil: Villena, Julio Cesar. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina |
Databáze: | OpenAIRE |
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