Glucose 6P Binds and Activates HlyIIR to Repress Bacillus cereus Haemolysin hlyII Gene Expression
| Autor: | Didier Rognan, Nalini Ramarao, Didier Lereclus, Elisabeth Guillemet, Seav-Ly Tran, Céline Cadot |
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| Přispěvatelé: | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, UMR 7200, Lab Therapeut Innovat, Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Innovation Thérapeutique (LIT), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
II GENE
[SDV]Life Sciences [q-bio] Bacillus cereus lcsh:Medicine PROTEIN Biochemistry Hemolysin Proteins Gene expression THURINGIENSIS Transcriptional regulation Biomacromolecule-Ligand Interactions RNA Processing Post-Transcriptional lcsh:Science 0303 health sciences Multidisciplinary biology Cell Death TOXIN GENE Hemolysin 3. Good health Bacterial Pathogens Host-Pathogen Interaction ENVIRONMENTAL-CONDITIONS Cereus BACTERIA Glucose-6-Phosphatase Medicine Bacterial and Foodborne Illness Research Article REGULATES EXPRESSION Programmed cell death Gastroenterology and Hepatology PORE Microbiology Immune system proteins 03 medical and health sciences Bacterial Proteins DNA-binding proteins Genetics Biology Microbial Pathogens 030304 developmental biology Gram Positive 030306 microbiology Cofactors Macrophages lcsh:R Proteins IRON-METABOLISM Gene Expression Regulation Bacterial biology.organism_classification Glucose Small Molecules VIRULENCE lcsh:Q Gene Function Bacteria |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2013, 8 (2), ⟨10.1371/journal.pone.0055085⟩ Plos One 2 (8), . (2013) PLoS ONE, Vol 8, Iss 2, p e55085 (2013) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0055085⟩ |
| Popis: | Bacillus cereus is a Gram-positive spore-forming bacterium causing food poisoning and serious opportunistic infections. These infections are characterized by bacterial accumulation despite the recruitment of phagocytic cells. We have previously shown that B. cereus Haemolysin II (HlyII) induces macrophage cell death by apoptosis. In this work, we investigated the regulation of the hlyII gene. We show that HlyIIR, the negative regulator of hlyII expression in B. cereus, is especially active during the early bacterial growth phase. We demonstrate that glucose 6P directly binds to HlyIIR and enhances its activity at a post-transcriptional level. Glucose 6P activates HlyIIR, increasing its capacity to bind to its DNA-box located upstream of the hlyII gene, inhibiting its expression. Thus, hlyII expression is modulated by the availability of glucose. As HlyII induces haemocyte and macrophage death, two cell types that play a role in the sequestration of nutrients upon infection, HlyII may induce host cell death to allow the bacteria to gain access to carbon sources that are essential components for bacterial growth. |
| Databáze: | OpenAIRE |
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