Yukmijihwang-tang protects against cyclophosphamide-induced reproductive toxicity
Autor: | Myung Sook Oh, Seong Kyu Park, Hyunsu Bae, Do Rim Kim, Mun Seog Chang, Wansu Park, Youngbuhm Huh |
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Rok vydání: | 2007 |
Předmět: |
Male
medicine.medical_specialty CAMP-Responsive Element Modulator Cyclophosphamide Side effect Gene Expression Biology Protective Agents Toxicology Cyclic AMP Response Element Modulator Lipid peroxidation chemistry.chemical_compound Internal medicine Testis medicine Animals Testosterone Rats Wistar education Antineoplastic Agents Alkylating Sperm motility Medicine East Asian Traditional education.field_of_study Plants Medicinal Sperm Count Plant Extracts Organ Size Rats Endocrinology chemistry Toxicity Sperm Motility Lipid Peroxidation Reproductive toxicity medicine.drug |
Zdroj: | Reproductive Toxicology. 24:365-370 |
ISSN: | 0890-6238 |
Popis: | A side effect of cyclophosphamide (CP), an alkylating agent widely used to treat tumors and autoimmune disorders, is the alteration of male reproductive function. Yukmijihwang-tang (YJT) is a multi-herbal medicinal formula that has been used in traditional Asian medicine to treat male reproductive problems. In this study, we investigated the effects of YJT on CP-induced reproductive toxicities in rat testes. Eight-week-old male Wistar rats were divided into three groups: vehicle-treated (control), CP-treated and CP + YJT-treated groups. CP was administered for the first 7 days (20 mg/kg/day, p.o.), and YJT was administered for 56 days consecutively (1.0 g/kg/day, p.o.). The CP-treated group showed significant decreases in the weight of the testes, epididymal sperm count and sperm motility compared to the control group, while the CP + YJT-treated group had significant increases for these variables compared to the CP-treated group. The enhancement of lipid peroxidation by CP in the rat testes was reduced by YJT treatment. CP diminished the expression of cAMP-responsive element modulator (CREM), a transcription factor that is highly expressed in male germ cells and is crucial to post-meiotic germ cell differentiation. YJT restored CREM at both the mRNA and protein levels. These results suggest that YJT has a protective effect against CP-induced reproductive toxicities by inhibiting the increases in lipid peroxidation and enhancing CREM expression. |
Databáze: | OpenAIRE |
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