Kinetics of interleukin-6 production after experimental infection of mice with Schistosoma mansoni
| Autor: | R. M. A. Khalil, R. Mailhammer, J. Moeller, A. A. Mohamed, S. Omran, P Dörmer, L. Hültner, A. Luz |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_treatment
Immunology Spleen Schistosomiasis Kidney Andrology Mice Immune system medicine Animals Immunology and Allergy Cytotoxic T cell RNA Messenger Interleukin 6 Cells Cultured Mice Inbred BALB C Granuloma biology Interleukin-6 Pokeweed mitogen Blotting Northern medicine.disease biology.organism_classification Schistosomiasis mansoni Stimulation Chemical medicine.anatomical_structure Cytokine Liver Pokeweed Mitogens biology.protein Female Schistosoma mansoni Research Article |
| Zdroj: | Immunology. 89:256-261 |
| ISSN: | 1365-2567 0019-2805 |
| Popis: | It has been reported that interleukin-6 (IL-6) is expressed in cells of acute inflammatory granulomas experimentally induced in mice by eggs of Schistosoma mansoni. Moreover, in vitro IL-6 was shown to enhance the cytotoxic activity of human platelets against larvae of S. mansoni. To elucidate further a proposed biological significance of this cytokine during the course of schistosomiasis, we studied the kinetics of IL-6 production and concomitantly performed a histopathological analysis of the livers in BALB/c mice subcutaneously infected with S. mansoni cercariae. Over a period of 24 weeks postinfection (p.i.) we monitored serum IL-6 levels, IL-6 production in vitro by pokeweed mitogen (PWM)-stimulated spleen cells as well as IL-6 mRNA expression in livers, spleens and kidneys. We found significantly elevated IL-6 levels in PWM-stimulated spleen cell-conditioned media (SCM) at weeks 6 to 20 p.i., peaking at week 10 p.i. In contrast, serum IL-6 concentrations started to rise not before week 8 but remained significantly elevated above normal control values until week 24 p.i. The time pattern of enhanced IL-6 mRNA expression detected in spleens and livers, but not in kidneys, as well as the rises of IL-6 in SCM and with a delay of 2 weeks in serum samples correlated with the onset of the egg-induced inflammatory reactions as well as the incidence and the number of the granulomas observed histopathologically in the livers of infected mice. Our data emphasize both a local and a systemic role of IL-6 in the host immune response following infection of mice with S. mansoni. |
| Databáze: | OpenAIRE |
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