Enteropathogenic Escherichia coli decreases the transepithelial electrical resistance of polarized epithelial monolayers
| Autor: | Sharon Ruschkowski, J.B. Kaper, B. Brett Finlay, M S Donnenberg, Ilan Rosenshine, C Canil |
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| Rok vydání: | 1993 |
| Předmět: |
Immunology
Biology digestive system Microbiology Cell junction Epithelium Cell Line Dogs Cell polarity Electric Impedance Escherichia coli medicine Animals Humans Enteropathogenic Escherichia coli Transcellular Transepithelial potential difference Tight junction Inulin Cell Polarity Epithelial Cells Actins Anti-Bacterial Agents Cell biology Intercellular Junctions Infectious Diseases medicine.anatomical_structure Paracellular transport Parasitology Research Article |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.61.7.2755-2762.1993 |
| Popis: | The mechanisms whereby enteropathogenic Escherichia coli (EPEC) causes diarrhea remain undefined. We found that EPEC caused a decrease in transepithelial electrical resistance across polarized monolayers of Caco-2 and MDCK epithelial cells. This occurred approximately 6 to 10 h after bacterial addition and was reversible if the monolayers were treated with tetracycline or gentamicin. Although significant alterations in host actin occurred beneath adherent EPEC, actin filaments supporting tight junctions were not noticeably affected in the epithelial cells, nor was the distribution of ZO-1, a tight junction protein. Despite the decrease in transepithelial electrical resistance, EPEC did not cause an increase in [3H]inulin penetration across MDCK monolayers. Unlike in the parental strain, mutations in any loci involved in adherence or formation of attaching and effacing lesions were unable to cause a decrease in transepithelial resistance. These data indicate that EPEC causes a decrease in transepithelial electrical resistance by disrupting a transcellular (intracellular) pathway rather than by disrupting intercellular tight junctions (paracellular) and that these disruptions occur only when attaching and effacing lesions are formed. |
| Databáze: | OpenAIRE |
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