Developmental Expression of Pulmonary Surfactant Protein D (SP-D)
| Autor: | Kevin Rust, Deena Parghi, Donald Chang, Anders Persson, Edmond C. Crouch, William Marienchek |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty Amniotic fluid Molecular Sequence Data Clinical Biochemistry Biology Immunoenzyme Techniques Embryonic and Fetal Development Affinity chromatography medicine Animals Amino Acid Sequence RNA Messenger Pulmonary surfactant protein D Lung Molecular Biology Glycoproteins Fetus Immunoperoxidase Surfactant protein D Pulmonary Surfactants Rats Inbred Strains Cell Biology Amniotic Fluid Pulmonary Surfactant-Associated Protein D Molecular biology Rats Staining medicine.anatomical_structure |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 5:13-18 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/ajrcmb/5.1.13 |
| Popis: | Surfactant protein D (SP-D) is a collagenous, surfactant-associated, carbohydrate-binding protein that is synthesized by pulmonary epithelial cells. In the present studies, we examined the expression of SP-D and SP-D mRNA during late fetal (day 17, 19, 21) and early postnatal (day 5) rat lung development using immunochemical, cell-free translation, and Northern hybridization assays. SP-D mRNA and immunoreactive SP-D protein were first detected in guanidine extracts of whole rat lung at 21 days of gestation and reached even higher concentrations during the postnatal period. Likewise, immunoperoxidase studies of rat lung using affinity-purified antibodies to SP-D showed no staining at day 17 or 19, but demonstrated strong cytoplasmic staining of cuboidal epithelial cells lining immature airspaces at day 21 and strong cytoplasmic staining of type II and nonciliated bronchiolar cells in adult lung. SP-D also appeared in amniotic fluid by day 21 and was partially purified by affinity chromatography on maltosyl-agarose under conditions used for the isolation of rat lung SP-D. These studies indicate that the production of SP-D is increased shortly prior to birth, and that the increases in total lung SP-D and SP-D mRNA are temporally correlated with SP-D secretion and the appearance of SP-D in amniotic fluid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|