Genomic Analysis of Rotavirus Species A Isolated from Swine, Amazon Region, Brazil
| Autor: | Kathleen V. S. de Araújo, Daniel S. de Camargo, Joana D'Arc Pereira Mascarenhas, Patrícia dos Santos Lobo, Luana da Silva Soares, Renato da Silva Bandeira, Mayara A. O Neves |
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| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| ISSN: | 2319-7706 2319-7692 |
| DOI: | 10.20546/ijcmas.2018.711.095 |
| Popis: | MS/SVS/Instituto Evandro Chagas Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil. Federal University of Par?. Center for Tropical Medicine. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. This present study has aimed to provide a molecular characterization of rotavirus species A (RVA) and to determine the frequency of RVC and RVH in fecal specimens from nonvaccinated piglets, across 5 farms in the metropolitan mesoregion of Bel?m, within the Amazon region, Brazil. In the previous study, porcine RVA was found in 9.9% (17/172) during the years 2008-2009. Using RT-PCR, Sanger nucleotide sequencing, and phylogenetic analyses, the entire genomes of 17 Brazilian porcine RVA strains were analyzed. The results indicated that all strains shared the same constellation, with the exception of the VP3 and NSP3 genes, which were M2 and T7 genotypes, respectively. The VP1, VP2, NSP2, NSP4 and NSP5 coding segments clustered into the Wa-like constellation; genotype 1, and VP7, VP4, VP6 and NSP1 genes were typically of porcine origin. RVA genes infecting pigs presented a high degree of nucleotide similarity to strains from other hosts, including humans. Little is known about the zoonotic potential of rotaviruses in Brazil, but the results of this study show dynamic interactions between human and porcine rotavirus strains and constitute an increase in knowledge on the diversity of RVA strains, in animal reservoirs circulating in the Amazon region. |
| Databáze: | OpenAIRE |
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