Microgravimetric analysis of nerve edema
| Autor: | Henry C. Powell, Michael L. Costello, Robert R. Myers |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Density gradient
Physiology Hexachlorophene Body water Cellular and Molecular Neuroscience Body Water Physiology (medical) Edema medicine Animals Desiccation Brain edema Chemistry Peripheral Nervous System Diseases Rats Inbred Strains Anatomy Sciatic Nerve Rats Female Neurology (clinical) Sciatic nerve medicine.symptom Densitometry Biomedical engineering medicine.drug |
| Zdroj: | Musclenerve. 5(4) |
| ISSN: | 0148-639X |
| Popis: | A technique for microgravimetric analysis of nerve edema was used to demonstrate increased water content in hexachlorophene neuropathy. The method was modified from one used previously to quantify brain edema by using density gradient columns prepared with two nonaqueous fluids of high and low specific gravity. This method revealed a wide separation between hexachlorophene-treated and control nerves, Gradient position was then related to specific gravity, which averaged 1.03426 in hexachlorophene-treated nerves versus 1.04921 for control nerves. This, in turn, was correlated with the percentage of water in the tissue. Water content of hexachlorophene-intoxicated nerves was approximately 10% greater than control nerves. A major advantage of this technique is its sensitivity in detecting edema in small tissue samples. |
| Databáze: | OpenAIRE |
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