Rac is involved in the interkinetic nuclear migration of cortical progenitor cells
| Autor: | Miyako Kimura, Wataru Ochiai, Akira Mizoguchi, Tomoko Ohdachi, Yuji Nishizawa, Takeshi Kawauchi, Rieko Kanda, Sayaka Minobe, Akira Sakakibara, Takaki Miyata, Sayaka Nakatani, Ryosuke Tadokoro |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
rac1 GTP-Binding Protein
Interkinetic nuclear migration Time Factors Neurogenesis Green Fluorescent Proteins RAC1 Biology Cerebral Ventricles Mice Organ Culture Techniques Cell Movement Pregnancy Animals Guanine Nucleotide Exchange Factors Vimentin Small GTPase T-Lymphoma Invasion and Metastasis-inducing Protein 1 Progenitor cell Amino Acids Cerebral Cortex Neurons Mice Inbred ICR General Neuroscience Stem Cells Cell Cycle Age Factors Gene Expression Regulation Developmental General Medicine Cell cycle Embryo Mammalian Neural stem cell Cell biology Neuroepithelial cell Protein Transport Electroporation Pyrimidines Immunology Aminoquinolines Female Cytokinesis |
| Zdroj: | Neuroscience research. 63(4) |
| ISSN: | 0168-0102 |
| Popis: | The small GTPase Rac regulates neuronal behavior, but whether it also functions in neural progenitor cells has not yet been explored. Here we report that Rac contributes to the regulation of nuclear migration in neocortical progenitor cells. Rac1 is expressed by progenitor cells in a unique spatiotemporal pattern. Cross-sectional immunohistochemical examination revealed intense Rac1 immunoreactivity at the ventricular surface. Similar staining patterns were obtained by immunofluorescence for a Rac-activator, Tiam1, and by reactions to detect the GTP-bound (active) form of Rac. En face inspection of the ventricular surface revealed that apical Rac1 localization was most frequent in M-phase cells, and the endfeet of cells in other cell cycle phases also showed apical Rac1 distribution at lower frequencies. To ask whether progenitor cell behavior prior to and during M phase is Rac-dependent, we monitored individual DiI-labeled progenitor cells live in the presence of a Rac inhibitor, NSC23766. We observed significantly retarded adventricular nuclear migration, as well as cytokinesis failures. Similar inhibitory effects were obtained by forced expression of a dominant-negative Rac1. These results suggest that Rac may play a role in interkinetic nuclear migration in the developing mouse brain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect