Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice
| Autor: | Austen Y. Chen, Scott R. Fry, T. K. S. Mukkur, Grant Daggard |
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| Rok vydání: | 2008 |
| Předmět: |
endocrine system
animal diseases chemical and pharmacologic phenomena Injections Intramuscular Immunoglobulin G DNA vaccination Mice Immune system Mycoplasma hyopneumoniae Antigen Vaccines DNA Animals Antigens Bacterial Mice Inbred BALB C Vaccines Synthetic General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health biochemical phenomena metabolism and nutrition biology.organism_classification Virology Antibodies Bacterial Lymphocyte Subsets Bacterial vaccine Infectious Diseases Humoral immunity Immunology Bacterial Vaccines Vaccines Subunit biology.protein bacteria Molecular Medicine Female Immunization Antibody |
| Zdroj: | Vaccine. 26(34) |
| ISSN: | 0264-410X |
| Popis: | Intramuscular immunization of mice with DNA cocktail vaccines, comprising potential protective antigens P36, P46, NrdF, and P97or P97R1 of Mycoplasma hyopneumoniae, induced strong Th1-polarized immune responses against each antigen, with only P46 eliciting a serum IgG response. Subcutaneous immunization with protein cocktail vaccines, surprisingly, induced both Th1-polarized immune response as well as antibody response whereas mice immunized with DNA cocktail vaccines followed by boosting with protein cocktail vaccines generated strong Th1-polarized and humoral immune responses. P97 was not recognized by serum antibodies from commercial bacterin-immunized mice indicating potential lack of expression of this important antigen in inactivated whole-cell vaccines. |
| Databáze: | OpenAIRE |
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