NrsZ : a novel, processed, nitrogen‐dependent, small non‐codingRNA that regulatesP seudomonas aeruginosa PAO 1 virulence
| Autor: | Nicolas Wenner, Alexandre Maes, Marta Cotado-Sampayo, Karine Lapouge |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Nitrogen
Virulence Factors Virulence Motility Swarming motility Biology medicine.disease_cause Microbiology chemistry.chemical_compound Bacterial Proteins Sigma factor medicine Research Articles Ecology Evolution Behavior and Systematics Pseudomonas aeruginosa Rhamnolipid RNA Gene Expression Regulation Bacterial beta-Galactosidase Bacterial Proteins/genetics Bacterial Proteins/metabolism Glycolipids/metabolism Nitrogen/metabolism Pseudomonas aeruginosa/pathogenicity Pseudomonas aeruginosa/physiology RNA Small Untranslated/chemistry RNA Small Untranslated/genetics Virulence/genetics Virulence Factors/genetics beta-Galactosidase/genetics beta-Galactosidase/metabolism chemistry Biochemistry RNA Small Untranslated bacteria rpoN Glycolipids |
| Zdroj: | Environmental Microbiology, vol. 16, no. 4, pp. 1053-1068 Environmental Microbiology |
| ISSN: | 1462-2920 1462-2912 |
| DOI: | 10.1111/1462-2920.12272 |
| Popis: | The opportunistic pathogen Pseudomonas aeruginosa PAO1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. Here, we report the discovery and characterization of a novel small non-coding RNA: NrsZ (nitrogen-regulated sRNA). We show that under nitrogen limitation, NrsZ is induced by the NtrB/C two-component system, an important regulator of nitrogen assimilation and P. aeruginosa's swarming motility, in concert with the alternative sigma factor RpoN. Furthermore, we demonstrate that NrsZ modulates P. aeruginosa motility by controlling the production of rhamnolipid surfactants, virulence factors notably needed for swarming motility. This regulation takes place through the post-transcriptional control of rhlA, a gene essential for rhamnolipids synthesis. Interestingly, we also observed that NrsZ is processed in three similar short modules, and that the first short module encompassing the first 60 nucleotides is sufficient for NrsZ regulatory functions. |
| Databáze: | OpenAIRE |
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