Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization
Autor: | Vanessa Uemura da Fonseca, Juliana Shimara Pires Ferrão, Paula de Carvalho Papa, Antenor Pereira Bonfim Neto, Liza Margareth Medeiros de Carvalho Sousa |
---|---|
Rok vydání: | 2019 |
Předmět: |
Male
Pathology medicine.medical_specialty Hind limbs Mouse Endothelium medicine.medical_treatment Vascular Endothelial Growth Factor C Stereology Hindlimb Revascularization Mice Animals Medicine Lymphangiogenesis Lymphatic Vessels Mice Inbred BALB C General Veterinary business.industry Original Articles Vascular Endothelial Growth Factor Receptor-3 MEDICINA REGENERATIVA medicine.anatomical_structure Lymphatic system Vascular endothelial growth factor C Lymph Node Excision Lymphatic disorders Immunohistochemistry Original Article Lymph business |
Zdroj: | Veterinary Medicine and Science Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
ISSN: | 2053-1095 |
DOI: | 10.1002/vms3.151 |
Popis: | Spontaneous lymphatic revascularization is a challenge and the establishment of new therapeutic strategies may improve life quality for patients suffering from lymphatic disorders. This study was designed to verify if VEGFC treatment improves lymphatic vascularization in a time‐dependent manner in mouse hindlimb (HL) after resection of the inguinal lymph node. Lymphatic vascular density (Vv) and length (Lv) were evaluated by stereology after immunohistochemistry. The control Group (CG) was not manipulated but received saline instead of VEGFC treatment. The surgery Group (SG) had the left inguinal lymph node resected but did not received VEGFC treatment. VEGFC Treated Group (TG) had the node resected and received VEGFC treatment. VEGFC and VEGFR3 local expression were assessed by qPCR. There was an effect of time over Vv and Lv in the SG and significant difference between CG and SG in the regions studied (proximal, medium and distal regions) of the left HL (LHL). The Lv showed significant difference between CG and SG only in the medium region. The Vv and the Lv for TG were higher than the other groups. VEGFC and VEGFR3 gene expression presented time effect in all regions of the LHL for SG and TG. Both VEGFC and VEGFR3 gene expression presented significant difference between CG and SG, between SG and TG and between CG and TG. This study showed significant decrease in lymphatic vascularization in the left hindlimb of mice after surgical removal of the inguinal lymph node and adjacent lymphatic vessels. Exogenous VEGFC could recover lymphatic vascularization through stimulating neolymphangiogenesis. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |