Single-cell analysis of cytokine production shows different immune profiles in multiple sclerosis patients with active or quiescent disease
| Autor: | Domenico Caputo, Rosella Cavaretta, Marina Saresella, Massimo Filippi, Mario Clerici, Sabrina Fossati, Livianna Speciale, Pasquale Ferrante, Stefania Ruzzante, Daria Trabattoni |
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| Přispěvatelé: | Clerici, M, Saresella, M, Trabattoni, D, Speciale, L, Fossati, S, Ruzzante, S, Cavaretta, R, Filippi, Massimo, Caputo, D, Ferrante, P. |
| Rok vydání: | 2001 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male medicine.medical_treatment CD14 Immunology Lipopolysaccharide Receptors CD8-Positive T-Lymphocytes In Vitro Techniques Peripheral blood mononuclear cell Enterotoxins Interferon-gamma Multiple Sclerosis Relapsing-Remitting Immune system Adjuvants Immunologic CD28 Antigens medicine Humans Immunology and Allergy Longitudinal Studies Immunity Cellular biology Interleukin-6 Tumor Necrosis Factor-alpha Multiple sclerosis Antibodies Monoclonal Myelin Basic Protein Interferon-beta Middle Aged medicine.disease Interleukin-12 Myelin basic protein Cytokine Neurology biology.protein Cytokines Interleukin-2 Female Tumor necrosis factor alpha Neurology (clinical) Antibody Interleukin-1 |
| Zdroj: | Journal of Neuroimmunology. 121:88-101 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/s0165-5728(01)00431-3 |
| Popis: | Peripheral blood mononuclear cells of multiple sclerosis (MS) patients were stimulated with myelin basic protein (MBP) together with anti-CD28 monoclonal antibody and staphylococcal enterotoxin B to optimize cytokine production by antigen-specific cells. Type 1 (IL-2, IL-12, IFNgamma) and pro-inflammatory (TNFalpha, IL-1beta, IL-6) cytokines were augmented in CD4+, CD8+, and CD14+ cells of acute MS patients and of patients undergoing disease reactivation. These cytokines were reduced in IFNbeta-treated and in stable MS patients; type 2 cytokines (IL-4, IL-10) were increased in these patients. Similar immune profiles are seen in MS patients in whom remission is naturally or pharmacologically (IFNbeta) achieved. Cytokine alterations are particularly evident in CD14+ cells, underlying their critical role in the modulation of the immune response. |
| Databáze: | OpenAIRE |
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