Chemotactic and Angiogenic Potential of Mineralized Collagen Scaffolds Functionalized with Naturally Occurring Bioactive Factor Mixtures to Stimulate Bone Regeneration

Autor: Anja Lode, Henriette Bretschneider, Stefan Rammelt, Michael Gelinsky, Mandy Quade, Corina Vater
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
medicine.medical_treatment
Gene Expression
Biocompatible Materials
02 engineering and technology
Umbilical vein
hypoxia-conditioned medium
bone regeneration
Biology (General)
Spectroscopy
Cells
Cultured
MSC secretome
Tube formation
Tissue Scaffolds
Chemistry
Chemotaxis
Cell migration
growth factor
General Medicine
Computer Science Applications
Cell biology
Adipose Tissue
Female
Collagen
adipose tissue extract
Adult
QH301-705.5
0206 medical engineering
bone substitute materials
Catalysis
Article
Cell Line
Inorganic Chemistry
03 medical and health sciences
Young Adult
medicine
Humans
Physical and Theoretical Chemistry
Bone regeneration
Molecular Biology
QD1-999
Biological Products
platelet lysates
Growth factor
Regeneration (biology)
Organic Chemistry
Mesenchymal stem cell
collagen scaffold
020601 biomedical engineering
030104 developmental biology
Bone Substitutes
Angiogenesis Inducing Agents
Biomarkers
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 11
International Journal of Molecular Sciences, Vol 22, Iss 5836, p 5836 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22115836
Popis: To develop cost-effective and efficient bone substitutes for improved regeneration of bone defects, heparin-modified mineralized collagen scaffolds were functionalized with concentrated, naturally occurring bioactive factor mixtures derived from adipose tissue, platelet-rich plasma and conditioned medium from a hypoxia-treated human bone marrow-derived mesenchymal stem cell line. Besides the analysis of the release kinetics of functionalized scaffolds, the bioactivity of the released bioactive factors was tested with regard to chemotaxis and angiogenic tube formation. Additionally, functionalized scaffolds were seeded with human bone marrow-derived mesenchymal stromal cells (hBM-MSC) and their osteogenic and angiogenic potential was investigated. The release of bioactive factors from the scaffolds was highest within the first 3 days. Bioactivity of the released factors could be confirmed for all bioactive factor mixtures by successful chemoattraction of hBM-MSC in a transwell assay as well as by the formation of prevascular structures in a 2D co-culture system of hBM-MSC and human umbilical vein endothelial cells. The cells seeded directly onto the functionalized scaffolds were able to express osteogenic markers and form tubular networks. In conclusion, heparin-modified mineralized collagen scaffolds could be successfully functionalized with naturally occurring bioactive factor mixtures promoting cell migration and vascularization.
Databáze: OpenAIRE
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