Chemotactic and Angiogenic Potential of Mineralized Collagen Scaffolds Functionalized with Naturally Occurring Bioactive Factor Mixtures to Stimulate Bone Regeneration
Autor: | Anja Lode, Henriette Bretschneider, Stefan Rammelt, Michael Gelinsky, Mandy Quade, Corina Vater |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Male medicine.medical_treatment Gene Expression Biocompatible Materials 02 engineering and technology Umbilical vein hypoxia-conditioned medium bone regeneration Biology (General) Spectroscopy Cells Cultured MSC secretome Tube formation Tissue Scaffolds Chemistry Chemotaxis Cell migration growth factor General Medicine Computer Science Applications Cell biology Adipose Tissue Female Collagen adipose tissue extract Adult QH301-705.5 0206 medical engineering bone substitute materials Catalysis Article Cell Line Inorganic Chemistry 03 medical and health sciences Young Adult medicine Humans Physical and Theoretical Chemistry Bone regeneration Molecular Biology QD1-999 Biological Products platelet lysates Growth factor Regeneration (biology) Organic Chemistry Mesenchymal stem cell collagen scaffold 020601 biomedical engineering 030104 developmental biology Bone Substitutes Angiogenesis Inducing Agents Biomarkers |
Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 11 International Journal of Molecular Sciences, Vol 22, Iss 5836, p 5836 (2021) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms22115836 |
Popis: | To develop cost-effective and efficient bone substitutes for improved regeneration of bone defects, heparin-modified mineralized collagen scaffolds were functionalized with concentrated, naturally occurring bioactive factor mixtures derived from adipose tissue, platelet-rich plasma and conditioned medium from a hypoxia-treated human bone marrow-derived mesenchymal stem cell line. Besides the analysis of the release kinetics of functionalized scaffolds, the bioactivity of the released bioactive factors was tested with regard to chemotaxis and angiogenic tube formation. Additionally, functionalized scaffolds were seeded with human bone marrow-derived mesenchymal stromal cells (hBM-MSC) and their osteogenic and angiogenic potential was investigated. The release of bioactive factors from the scaffolds was highest within the first 3 days. Bioactivity of the released factors could be confirmed for all bioactive factor mixtures by successful chemoattraction of hBM-MSC in a transwell assay as well as by the formation of prevascular structures in a 2D co-culture system of hBM-MSC and human umbilical vein endothelial cells. The cells seeded directly onto the functionalized scaffolds were able to express osteogenic markers and form tubular networks. In conclusion, heparin-modified mineralized collagen scaffolds could be successfully functionalized with naturally occurring bioactive factor mixtures promoting cell migration and vascularization. |
Databáze: | OpenAIRE |
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