Peutz-Jeghers polyps, dysplasia, and K-ras codon 12 mutations
| Autor: | Stanley R. Hamilton, M.L.F. van Velthuysen, J. H.P. Wilson, Francis M. Giardiello, G. J. A. Offerhaus, Robbert J.C. Slebos, Anne Marie Westerman, Mark M. Entius, M. M. Polak |
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| Přispěvatelé: | Faculteit der Geneeskunde, Other departments, Internal Medicine, Pathology |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Pathology medicine.medical_specialty Colorectal cancer Glycine Peutz-Jeghers Syndrome Peutz–Jeghers syndrome Malignancy medicine.disease_cause Polymerase Chain Reaction medicine Carcinoma Humans Point Mutation Codon skin and connective tissue diseases Cancer Mutation business.industry Point mutation Gastroenterology Valine medicine.disease digestive system diseases Genes ras Dysplasia Cancer research Autoradiography Lentiginosis business |
| Zdroj: | Gut, 41, 320-322. BMJ Publishing Group Gut, 41(3), 320-322. BMJ Publishing Group |
| ISSN: | 0017-5749 |
| Popis: | Background—Peutz-Jeghers syndrome (PJS) is a rare, autosomal dominant, polyposis syndrome, associated with an increased risk of gastrointestinal and extragastrointestinal malignancy. Occasionally dysplasia occurs in PJS polyps.Aims—In colorectal carcinomas, mutations in codon 12 of the K-ras oncogene are common and are found at similar frequency in precursor adenomas. Therefore, K-rascodon 12 point mutations in PJS polyps were evaluated.Materials and methods—Fifty two PJS polyps, including four with dysplasia, collected from 19 patients with PJS, were analysed for mutations in the K-ras codon 12 by a mutant enriched polymerase chain reaction procedure, followed by allele specific oligodeoxynucleotide hybridisation.Results—A K-ras codon 12 mutation was identified in one colonic polyp with dysplasia. The mutation was found in the non-neoplastic epithelial cells and not in the dysplastic component of the polyp.Conclusions—K-ras codon 12 point mutations are very rare in PJS polyps, by contrast with colorectal adenomas. The findings support previous evidence that there seems to be no intrinsic relation between K-ras codon 12 mutation and dysplasia. |
| Databáze: | OpenAIRE |
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